ReferenceID 3979
Lentil lectin derived from Lens culinaris exhibit broad antiviral activities against SARS-CoV-2 variants
Emerg Microbes Infect
The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutated continuously and newly emerging variants escape from antibody-mediated neutralization raised great concern. S protein is heavi
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- Reference Id
- 3979
- Evidence Id
- 20569
- Core Evidence Id
- 20569
- Source Reference Id
- 1234
- Herb2 Reference Id
- HBREF002031
- Subject Paper Key
- HERB000492_34278967
- Pubmed Id
- 34278967
- Doi
- 10.1080/22221751.2021.1957720
- Paper Title
- Lentil lectin derived from Lens culinaris exhibit broad antiviral activities against SARS-CoV-2 variants
- Paper Abstract
- The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutated continuously and newly emerging variants escape from antibody-mediated neutralization raised great concern. S protein is heavily glycosylated and the glycosylation sites are relatively conserved, thus glycans on S protein surface could be a target for the development of anti-SARS-CoV-2 strategies against variants. Here, we collected 12 plant-derived lectins with different carbohydrate specificity and evaluated their anti-SARS-CoV-2 activity against mutant strains and epidemic variants using a pseudovirus-based neutralization assay. The Lens culinaris-derived lentil lectin which specifically bind to oligomannose-type glycans and GlcNAc at the non-reducing end terminus showed most potent and broad antiviral activity against a panel of mutant strains and variants, including the artificial mutants at N-/O-linked glycosylation site, natural existed amino acid mutants, as well as the epidemic variants B.1.1.7, B.1.351, and P.1. Lentil lectin also showed antiviral activity against SARS-CoV and MERS-CoV. We found lentil lectin could block the binding of ACE2 to S trimer and inhibit SARS-CoV-2 at the early steps of infection. Using structural information and determined N-glycan profile of S trimer, taking together with the carbohydrate specificity of lentil lectin, we provide a basis for the observed broad spectrum anti-SARS-CoV-2 activity. Lentil lectin showed weak haemagglutination activity at 1 mg/mL and no cytotoxicity activity, and no weight loss was found in single injection mouse experiment. This report provides the first evidence that lentil lectin strongly inhibit infection of SARS-COV-2 variants, which should provide valuable insights for developing future anti-SARS-CoV-2 strategies.
- Journal
- Emerg Microbes Infect
- Publish Year
- 2021
- Experiment Subject
- mouse; lentil
- Experiment Type
- Others
- Phenotype Related
- Acute Respiratory Syndrome Coronavirus 2; Sars-cov-2
- Paper Title Cn
- Paper Title En
- Lentil lectin derived from Lens culinaris exhibit broad antiviral activities against SARS-CoV-2 variants
- Bilingual Status
- semi_complete