ReferenceID 3957
Salvianolic acid B suppresses maturation of human monocyte-derived dendritic cells by activating PPARγ
Br J Pharmacol
BACKGROUND AND PURPOSE: Salvianolic acid B (Sal B), a water-soluble antioxidant derived from a Chinese medicinal herb, is known to be effective in the prevention of atherosclerosis. Here, we tested the hypothesis that th
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- Reference Id
- 3957
- Evidence Id
- 20547
- Core Evidence Id
- 20547
- Source Reference Id
- 1192
- Herb2 Reference Id
- HBREF001989
- Subject Paper Key
- HBIN042901_21649636
- Pubmed Id
- 21649636
- Doi
- 10.1111/j.1476-5381.2011.01518.x
- Paper Title
- Salvianolic acid B suppresses maturation of human monocyte-derived dendritic cells by activating PPARγ
- Paper Abstract
- BACKGROUND AND PURPOSE: Salvianolic acid B (Sal B), a water-soluble antioxidant derived from a Chinese medicinal herb, is known to be effective in the prevention of atherosclerosis. Here, we tested the hypothesis that the anti-atherosclerotic effect of Sal B might be mediated by suppressing maturation of human monocyte-derived dendritic cells (h-monDC). EXPERIMENTAL APPROACH: h-monDC were derived by incubating purified human monocytes with GM-CSF and IL-4. h-monDC were pre-incubated with or without Sal B and stimulated by oxidized low-density lipoprotein (ox-LDL) in the presence or absence of PPARγ siRNA. Expression of h-monDC membrane molecules (CD40, CD86, CD1a, HLA-DR) were analysed by FACS, cytokines were measured by elisa and the TLR4-associated signalling pathway was determined by Western blotting. KEY RESULTS: Ox-LDL promoted h-monDC maturation, stimulated CD40, CD86, CD1a, HLA-DR expression and IL-12, IL-10, TNF-α production; and up-regulated TLR4 signalling. These effects were inhibited by Sal B. Sal B also triggered PPARγ activation and promoted PPARγ nuclear translocation, attenuated ox-LDL-induced up-regulation of TLR4 and myeloid differentiation primary-response protein 88 and inhibited the downstream p38-MAPK signalling cascade. Knocking down PPARγ with the corresponding siRNA blocked these effects of Sal B. CONCLUSIONS AND IMPLICATIONS: Our data suggested that Sal B effectively suppressed maturation of h-monDC induced by ox-LDL through PPARγ activation.
- Journal
- Br J Pharmacol
- Publish Year
- 2011
- Experiment Subject
- purified human monocytes
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Salvianolic acid B suppresses maturation of human monocyte-derived dendritic cells by activating PPARγ
- Bilingual Status
- semi_complete