ReferenceID 3855
Anti-tumor effects of Atractylenolide I on bladder cancer cells
J Exp Clin Cancer Res
BACKGROUND: Atractylenolide I (ATR-1), an active component of Rhizoma Atractylodis Macrocephalae, possesses cytotoxicity against various carcinomas. However, little is known about the effects of ATR-1on bladder cancer. I
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Record Fields
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- Reference Id
- 3855
- Evidence Id
- 20445
- Core Evidence Id
- 20445
- Source Reference Id
- 997
- Herb2 Reference Id
- HBREF001710
- Subject Paper Key
- HBIN017285_26931119
- Pubmed Id
- 26931119
- Doi
- 10.1186/s13046-016-0312-4
- Paper Title
- Anti-tumor effects of Atractylenolide I on bladder cancer cells
- Paper Abstract
- BACKGROUND: Atractylenolide I (ATR-1), an active component of Rhizoma Atractylodis Macrocephalae, possesses cytotoxicity against various carcinomas. However, little is known about the effects of ATR-1on bladder cancer. In the present study, the anti-tumor activity of ATR-1 was examined on bladder cancer cells both in vivo and in vitro. METHODS: MTT assay was used to assess the cytotoxic effect of ATR-1. Cell cycle distribution and apoptosis levels were evaluated using flow cytometry. Western blotting assay was applied to measure the levels of proteins associated with the apoptotic pathway, cell cycle progression and PI3K/Akt/mTOR signaling pathway. Tumor models in nude mice were induced by injection of T-24 and 253J human bladder cancer cells. RESULTS: ATR-1 inhibited bladder cancer cell proliferation, arrested cell cycle in G2/M phase through up-regulation of p21 and down-regulation of cyclin B1, CDK1 and Cdc25c. Meanwhile, ATR-1 also triggered cellular apoptosis depending on the activation of mitochondrial apoptotic pathway. Mechanism investigation indicated that ATR-1 exerts its anti-tumor effect also relies on the inhibition of PI3K/Akt/mTOR signaling pathway. Finally, mice studies showed that ATR-1 blocked the T-24 or 253J-induced xenograft tumor growth without noticeable toxicity. CONCLUSIONS: ATR-1 may be served as a potential therapeutic agent for the treatment of bladder cancer.
- Journal
- J Exp Clin Cancer Res
- Publish Year
- 2016
- Experiment Subject
- bladder cancer cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Bladder Cancer
- Paper Title Cn
- Paper Title En
- Anti-tumor effects of Atractylenolide I on bladder cancer cells
- Bilingual Status
- semi_complete