ReferenceID 3729
Phosphorylation-independent mTORC1 inhibition by the autophagy inducer Rottlerin
Cancer Lett
We recently found that Rottlerin not only inhibits proliferation but also causes Bcl-2- and Beclin 1-independent autophagic death in apoptosis-resistant breast adenocarcinoma MCF-7 cells. Having excluded a role for cano
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 3729
- Evidence Id
- 20319
- Core Evidence Id
- 20319
- Source Reference Id
- 736
- Herb2 Reference Id
- HBREF001269
- Subject Paper Key
- HBIN042467_25661734
- Pubmed Id
- 25661734
- Doi
- 10.1016/j.canlet.2015.01.040
- Paper Title
- Phosphorylation-independent mTORC1 inhibition by the autophagy inducer Rottlerin
- Paper Abstract
- We recently found that Rottlerin not only inhibits proliferation but also causes Bcl-2- and Beclin 1-independent autophagic death in apoptosis-resistant breast adenocarcinoma MCF-7 cells. Having excluded a role for canonical signaling pathways, the current study was aimed to investigate the contribution of the AMPK/mTOR axis in autophagy induction and to search for the upstream signaling molecules potentially targeted by Rottlerin. Using several enzyme inhibitors, Western blotting analysis, mTOR siRNA and pull down assay, we demonstrate that the Rottlerin-triggered autophagy is mediated by inhibition of mTORC1 activity through a novel AMPK and mTORC1 phosphorylation-independent mechanism, likely mediated by the direct interaction between Rottlerin and mTOR.
- Journal
- Cancer Lett
- Publish Year
- 2015
- Experiment Subject
- apoptosis-resistant breast adenocarcinoma mcf-7 cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Phosphorylation-independent mTORC1 inhibition by the autophagy inducer Rottlerin
- Bilingual Status
- semi_complete