ReferenceID 3609
HIV-1 infection of human macrophages directly induces viperin which inhibits viral production
Blood
Macrophages are key target cells for HIV-1. HIV-1(BaL) induced a subset of interferon-stimulated genes in monocyte-derived macrophages (MDMs), which differed from that in monocyte-derived dendritic cells and CD4 T cells,
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 3609
- Evidence Id
- 20199
- Core Evidence Id
- 20199
- Source Reference Id
- 519
- Herb2 Reference Id
- HBREF000870
- Subject Paper Key
- HBIN026385_22677126
- Pubmed Id
- 22677126
- Doi
- 10.1182/blood-2012-01-407395
- Paper Title
- HIV-1 infection of human macrophages directly induces viperin which inhibits viral production
- Paper Abstract
- Macrophages are key target cells for HIV-1. HIV-1(BaL) induced a subset of interferon-stimulated genes in monocyte-derived macrophages (MDMs), which differed from that in monocyte-derived dendritic cells and CD4 T cells, without inducing any interferons. Inhibition of type I interferon induction was mediated by HIV-1 inhibition of interferon-regulated factor (IRF3) nuclear translocation. In MDMs, viperin was the most up-regulated interferon-stimulated genes, and it significantly inhibited HIV-1 production. HIV-1 infection disrupted lipid rafts via viperin induction and redistributed viperin to CD81 compartments, the site of HIV-1 egress by budding in MDMs. Exogenous farnesol, which enhances membrane protein prenylation, reversed viperin-mediated inhibition of HIV-1 production. Mutagenesis analysis in transfected cell lines showed that the internal S-adenosyl methionine domains of viperin were essential for its antiviral activity. Thus viperin may contribute to persistent noncytopathic HIV-1 infection of macrophages and possibly to biologic differences with HIV-1-infected T cells.
- Journal
- Blood
- Publish Year
- 2012
- Experiment Subject
- macrophages (mdms)
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- HIV-1 infection of human macrophages directly induces viperin which inhibits viral production
- Bilingual Status
- semi_complete