ReferenceID 3584
Hippocampal histone acetylation regulates object recognition and the estradiol-induced enhancement of object recognition
J Neurosci
Histone acetylation has recently been implicated in learning and memory processes, yet necessity of histone acetylation for such processes has not been demonstrated using pharmacological inhibitors of histone acetyltrans
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Record Fields
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- Reference Id
- 3584
- Evidence Id
- 20174
- Core Evidence Id
- 20174
- Source Reference Id
- 468
- Herb2 Reference Id
- HBREF000799
- Subject Paper Key
- HBIN027349_22396409
- Pubmed Id
- 22396409
- Doi
- 10.1523/JNEUROSCI.5819-11.2012
- Paper Title
- Hippocampal histone acetylation regulates object recognition and the estradiol-induced enhancement of object recognition
- Paper Abstract
- Histone acetylation has recently been implicated in learning and memory processes, yet necessity of histone acetylation for such processes has not been demonstrated using pharmacological inhibitors of histone acetyltransferases (HATs). As such, the present study tested whether garcinol, a potent HAT inhibitor in vitro, could impair hippocampal memory consolidation and block the memory-enhancing effects of the modulatory hormone 17β-estradiol E2. We first showed that bilateral infusion of garcinol (0.1, 1, or 10 μg/side) into the dorsal hippocampus (DH) immediately after training impaired object recognition memory consolidation in ovariectomized female mice. A behaviorally effective dose of garcinol (10 μg/side) also significantly decreased DH HAT activity. We next examined whether DH infusion of a behaviorally subeffective dose of garcinol (1 ng/side) could block the effects of DH E2 infusion on object recognition and epigenetic processes. Immediately after training, ovariectomized female mice received bilateral DH infusions of vehicle, E2 (5 μg/side), garcinol (1 ng/side), or E2 plus garcinol. Forty-eight hours later, garcinol blocked the memory-enhancing effects of E2. Garcinol also reversed the E2-induced increase in DH histone H3 acetylation, HAT activity, and levels of the de novo methyltransferase DNMT3B, as well as the E2-induced decrease in levels of the memory repressor protein histone deacetylase 2. Collectively, these findings suggest that histone acetylation is critical for object recognition memory consolidation and the beneficial effects of E2 on object recognition. Importantly, this work demonstrates that the role of histone acetylation in memory processes can be studied using a HAT inhibitor.
- Journal
- J Neurosci
- Publish Year
- 2012
- Experiment Subject
- ovariectomized female mice
- Experiment Type
- Animal Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Hippocampal histone acetylation regulates object recognition and the estradiol-induced enhancement of object recognition
- Bilingual Status
- semi_complete