ReferenceID 3527
Inhibition of autophagy prevents cadmium-induced prostate carcinogenesis
Br J Cancer
BACKGROUND: Cadmium, an established carcinogen, is a risk factor for prostate cancer. Induction of autophagy is a prerequisite for cadmium-induced transformation and metastasis. The ability of Psoralidin (Pso), a non-tox
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Record Fields
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- Reference Id
- 3527
- Evidence Id
- 20117
- Core Evidence Id
- 20117
- Source Reference Id
- 358
- Herb2 Reference Id
- HBREF000651
- Subject Paper Key
- HBIN041131_28588318
- Pubmed Id
- 28588318
- Doi
- 10.1038/bjc.2017.143
- Paper Title
- Inhibition of autophagy prevents cadmium-induced prostate carcinogenesis
- Paper Abstract
- BACKGROUND: Cadmium, an established carcinogen, is a risk factor for prostate cancer. Induction of autophagy is a prerequisite for cadmium-induced transformation and metastasis. The ability of Psoralidin (Pso), a non-toxic, orally bioavailable compound to inhibit cadmium-induced autophagy to prevent prostate cancer was investigated. METHODS: Psoralidin was studied using cadmium-transformed prostate epithelial cells (CTPE), which exhibit high proliferative, invasive and colony forming abilities. Gene and protein expression were evaluated by qPCR, western blot, immunohistochemistry and immunofluorescence. Xenograft models were used to study the chemopreventive effects in vivo. RESULTS: Cadmium-transformed prostate epithelial cells were treated with Pso resulting in growth inhibition, without causing toxicity to normal prostate epithelial cells (RWPE-1). Psoralidin-treatment of CTPE cells inhibited the expression of Placenta Specific 8, a lysosomal protein essential for autophagosome and autolysosome fusion, which resulted in growth inhibition. Additionally, Pso treatment caused decreased expression of pro-survival signalling proteins, NFκB and Bcl2, and increased expression of apoptotic genes. In vivo, Pso effectively suppressed CTPE xenografts growth, without any observable toxicity. Tumours from Pso-treated animals showed decreased autophagic morphology, mesenchymal markers expression and increased epithelial protein expression. CONCLUSIONS: These results confirm that inhibition of autophagy by Pso plays an important role in the chemoprevention of cadmium-induced prostate carcinogenesis.
- Journal
- Br J Cancer
- Publish Year
- 2017
- Experiment Subject
- cadmium-transformed prostate epithelial cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Inhibition of autophagy prevents cadmium-induced prostate carcinogenesis
- Bilingual Status
- semi_complete