ReferenceID 3513
Oxidative stress modulates the cytokine response of differentiated Th17 and Th1 cells
Free Radic Biol Med
Reactive oxygen species (ROS) signaling is critical in T helper (Th) cell differentiation; however its role in differentiated Th cell functions is unclear. In this study, we investigated the role of oxidative stress on t
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Record Fields
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- Reference Id
- 3513
- Evidence Id
- 20103
- Core Evidence Id
- 20103
- Source Reference Id
- 333
- Herb2 Reference Id
- HBREF000618
- Subject Paper Key
- HBIN040266_27567538
- Pubmed Id
- 27567538
- Doi
- 10.1016/j.freeradbiomed.2016.08.026
- Paper Title
- Oxidative stress modulates the cytokine response of differentiated Th17 and Th1 cells
- Paper Abstract
- Reactive oxygen species (ROS) signaling is critical in T helper (Th) cell differentiation; however its role in differentiated Th cell functions is unclear. In this study, we investigated the role of oxidative stress on the effector functions of in vitro differentiated mouse Th17 and Th1 cells or CD4+ T cells from patients with Rheumatoid Arthritis using pro-oxidants plumbagin (PB) and hydrogen peroxide. We found that in mouse Th cells, non-toxic concentration of pro-oxidants inhibited reactivation induced expression of IL-17A in Th17 and IFN-γ in Th1 cells by reducing the expression of their respective TFs, RORγt and T-bet. Interestingly, in both the subsets, PB increased the expression of IL-4 by enhancing reactivation induced ERK1/2 phosphorylation. We further investigated the cytokine modulatory effect of PB on CD4+ T cells isolated from PBMCs of patients with Rheumatoid Arthritis, a well-known Th17 and or Th1 mediated disease. In human CD4+ T cells from Rheumatoid Arthritis patients, PB reduced the frequencies of IL-17A+ (Th17), IFN-γ+ (Th1) and IL-17A+/IFN-γ+ (Th17/1) cells and also inhibited the production of pro-inflammatory cytokines TNF-α and IL-6. N-Acetyl Cysteine (NAC) an antioxidant completely reversed PB mediated cytokine modulatory effects in both mouse and human cells indicating a direct role for ROS. Together our data suggest that oxidative microenvironment can alter cytokine response of terminally differentiated cells and thus altering intracellular ROS could be a potential way to target Th17 and Th1 cells in autoimmune disorders.
- Journal
- Free Radic Biol Med
- Publish Year
- 2016
- Experiment Subject
- in vitro differentiated mouse th17 and th1 cells or cd4+ t cells from patients with rheumatoid arthritis
- Experiment Type
- Cell Experiment
- Phenotype Related
- Autoimmune Disorders
- Paper Title Cn
- Paper Title En
- Oxidative stress modulates the cytokine response of differentiated Th17 and Th1 cells
- Bilingual Status
- semi_complete