ReferenceID 3503

Parthenolide inhibits nociception and neurogenic vasodilatation in the trigeminovascular system by targeting the TRPA1 channel

Pain

Although feverfew has been used for centuries to treat pain and headaches and is recommended for migraine treatment, the mechanism for its protective action remains unknown. Migraine is triggered by calcitonin gene-rela

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Reference Id
3503
Evidence Id
20093
Core Evidence Id
20093
Source Reference Id
306
Herb2 Reference Id
HBREF000564
Subject Paper Key
HBIN038876_23933184
Pubmed Id
23933184
Doi
10.1016/j.pain.2013.08.002
Paper Title
Parthenolide inhibits nociception and neurogenic vasodilatation in the trigeminovascular system by targeting the TRPA1 channel
Paper Abstract
Although feverfew has been used for centuries to treat pain and headaches and is recommended for migraine treatment, the mechanism for its protective action remains unknown. Migraine is triggered by calcitonin gene-related peptide (CGRP) release from trigeminal neurons. Peptidergic sensory neurons express a series of transient receptor potential (TRP) channels, including the ankyrin 1 (TRPA1) channel. Recent findings have identified agents either inhaled from the environment or produced endogenously that are known to trigger migraine or cluster headache attacks, such as TRPA1 simulants. A major constituent of feverfew, parthenolide, may interact with TRPA1 nucleophilic sites, suggesting that feverfew's antimigraine effect derives from its ability to target TRPA1. We found that parthenolide stimulates recombinant (transfected cells) or natively expressed (rat/mouse trigeminal neurons) TRPA1, where it, however, behaves as a partial agonist. Furthermore, in rodents, after initial stimulation, parthenolide desensitizes the TRPA1 channel and renders peptidergic TRPA1-expressing nerve terminals unresponsive to any stimulus. This effect of parthenolide abrogates nociceptive responses evoked by stimulation of peripheral trigeminal endings. TRPA1 targeting and neuronal desensitization by parthenolide inhibits CGRP release from trigeminal neurons and CGRP-mediated meningeal vasodilatation, evoked by either TRPA1 agonists or other unspecific stimuli. TRPA1 partial agonism, together with desensitization and nociceptor defunctionalization, ultimately resulting in inhibition of CGRP release within the trigeminovascular system, may contribute to the antimigraine effect of parthenolide.
Journal
Pain
Publish Year
2013
Experiment Subject
ype ( trpa1+/+) or trpa1- deficient (trpa1-/-) mice, or sprague-dawley rats (male, harlan laboratories),human embryonic kidney (hek293) cells stably transfected with the cdna of human trpv1 (htrpv1-hek293) or with the cdna of human trpa1 (htrpa1-hek293) and naive hek293 cells
Experiment Type
Animal & Cell Experiment
Phenotype Related
Migraine
Paper Title Cn
Paper Title En
Parthenolide inhibits nociception and neurogenic vasodilatation in the trigeminovascular system by targeting the TRPA1 channel
Bilingual Status
semi_complete