ReferenceID 3455

Cucurbitacin D Reprograms Glucose Metabolic Network in Prostate Cancer

Cancers (Basel)

Prostate cancer (PrCa) metastasis is the major cause of mortality and morbidity among men. Metastatic PrCa cells are typically adopted for aberrant glucose metabolism. Thus, chemophores that reprogram altered glucose met

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Reference Id
3455
Evidence Id
20045
Core Evidence Id
20045
Source Reference Id
238
Herb2 Reference Id
HBREF000448
Subject Paper Key
HBIN021848_30875788
Pubmed Id
30875788
Doi
10.3390/cancers11030364
Paper Title
Cucurbitacin D Reprograms Glucose Metabolic Network in Prostate Cancer
Paper Abstract
Prostate cancer (PrCa) metastasis is the major cause of mortality and morbidity among men. Metastatic PrCa cells are typically adopted for aberrant glucose metabolism. Thus, chemophores that reprogram altered glucose metabolic machinery in cancer cells can be useful agent for the repression of PrCa metastasis. Herein, we report that cucurbitacin D (Cuc D) effectively inhibits glucose uptake and lactate production in metastatic PrCa cells via modulating glucose metabolism. This metabolic shift by Cuc D was correlated with decreased expression of GLUT1 by its direct binding as suggested by its proficient molecular docking (binding energy -8.5 kcal/mol). Cuc D treatment also altered the expression of key oncogenic proteins and miR-132 that are known to be involved in glucose metabolism. Cuc D (0.1 to 1 M) treatment inhibited tumorigenic and metastatic potential of human PrCa cells via inducing apoptosis and cell cycle arrest in G2/M phase. Cuc D treatment also showed inhibition of tumor growth in PrCa xenograft mouse model with concomitant decrease in the expression of GLUT1, PCNA and restoration of miR-132. These results suggest that Cuc D is a novel modulator of glucose metabolism and could be a promising therapeutic modality for the attenuation of PrCa metastasis.
Journal
Cancers (Basel)
Publish Year
2019
Experiment Subject
human prca cells
Experiment Type
Cell Experiment
Phenotype Related
Prostate Cancer
Paper Title Cn
Paper Title En
Cucurbitacin D Reprograms Glucose Metabolic Network in Prostate Cancer
Bilingual Status
semi_complete