ReferenceID 3439
Regulation of the sperm calcium channel CatSper by endogenous steroids and plant triterpenoids
Proc Natl Acad Sci U S A
The calcium channel of sperm (CatSper) is essential for sperm hyperactivated motility and fertility. The steroid hormone progesterone activates CatSper of human sperm via binding to the serine hydrolase ABHD2. However, s
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 3439
- Evidence Id
- 20029
- Core Evidence Id
- 20029
- Source Reference Id
- 185
- Herb2 Reference Id
- HBREF000372
- Subject Paper Key
- HBIN033751_28507119
- Pubmed Id
- 28507119
- Doi
- 10.1073/pnas.1700367114
- Paper Title
- Regulation of the sperm calcium channel CatSper by endogenous steroids and plant triterpenoids
- Paper Abstract
- The calcium channel of sperm (CatSper) is essential for sperm hyperactivated motility and fertility. The steroid hormone progesterone activates CatSper of human sperm via binding to the serine hydrolase ABHD2. However, steroid specificity of ABHD2 has not been evaluated. Here, we explored whether steroid hormones to which human spermatozoa are exposed in the male and female genital tract influence CatSper activation via modulation of ABHD2. The results show that testosterone, estrogen, and hydrocortisone did not alter basal CatSper currents, whereas the neurosteroid pregnenolone sulfate exerted similar effects as progesterone, likely binding to the same site. However, physiological concentrations of testosterone and hydrocortisone inhibited CatSper activation by progesterone. Additionally, testosterone antagonized the effect of pregnenolone sulfate. We have also explored whether steroid-like molecules, such as the plant triterpenoids pristimerin and lupeol, affect sperm fertility. Interestingly, both compounds competed with progesterone and pregnenolone sulfate and significantly reduced CatSper activation by either steroid. Furthermore, pristimerin and lupeol considerably diminished hyperactivation of capacitated spermatozoa. These results indicate that (i) pregnenolone sulfate together with progesterone are the main steroids that activate CatSper and (ii) pristimerin and lupeol can act as contraceptive compounds by averting sperm hyperactivation, thus preventing fertilization.
- Journal
- Proc Natl Acad Sci U S A
- Publish Year
- 2017
- Experiment Subject
- Experiment Type
- Cell Experiment
- Phenotype Related
- Sperm Hyperactivated Motility
- Paper Title Cn
- Paper Title En
- Regulation of the sperm calcium channel CatSper by endogenous steroids and plant triterpenoids
- Bilingual Status
- semi_complete