ReferenceID 3438
Leonurine (SCM-198) attenuates myocardial fibrotic response via inhibition of NADPH oxidase 4
Free Radic Biol Med
In our previous studies, we have reported that leonurine, a plant phenolic alkaloid in Herba leonuri, exerted cardioprotective properties in a number of preclinical experiments. Herein, we investigated the roles and the
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Record Fields
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- Reference Id
- 3438
- Evidence Id
- 20028
- Core Evidence Id
- 20028
- Source Reference Id
- 184
- Herb2 Reference Id
- HBREF000370
- Subject Paper Key
- HBIN032907_23127783
- Pubmed Id
- 23127783
- Doi
- 10.1016/j.freeradbiomed.2012.10.555
- Paper Title
- Leonurine (SCM-198) attenuates myocardial fibrotic response via inhibition of NADPH oxidase 4
- Paper Abstract
- In our previous studies, we have reported that leonurine, a plant phenolic alkaloid in Herba leonuri, exerted cardioprotective properties in a number of preclinical experiments. Herein, we investigated the roles and the possible mechanisms of leonurine for reducing fibrotic responses in angiotensin II (Ang II)-stimulated primary neonatal rat cardiac fibroblasts and post-myocardial infarction (MI) rats. In in vitro experiments performed in neonatal rat cardiac fibroblasts, leonurine (10-20 μM) pretreatment attenuated Ang II-induced activation of extracellular signal-regulated kinase 1/2, production of intracellular reactive oxygen species (ROS), expression and activity of matrix metalloproteinase (MMP)-2/9, and expression of α-smooth muscle actin and types I and III collagen. A small interfering RNA-mediated knockdown strategy for NADPH oxidase 4 (Nox4) revealed that Nox4 was required for Ang II-induced activation of cardiac fibroblasts. In vivo studies using a post-MI model in rats indicated that administration of leonurine inhibited myocardial fibrosis while reducing cardiac Nox4 expression, ROS production, NF-κB activation, and plasma MMP-2 activity. In conclusion, our results provide the first evidence that leonurine could prevent cardiac fibrosis and the activation of cardiac fibroblasts partly through modulation of a Nox4-ROS pathway.
- Journal
- Free Radic Biol Med
- Publish Year
- 2013
- Experiment Subject
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Leonurine (SCM-198) attenuates myocardial fibrotic response via inhibition of NADPH oxidase 4
- Bilingual Status
- semi_complete