ReferenceID 3382
Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis
Cell Death Dis
Glioma is the most common and malignant form of primary brain tumour, and is characterised by high proliferation and extensive invasion and neurological destruction. Demethylzeylasteral (T-96), which is extracted from Tr
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Record Fields
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- Reference Id
- 3382
- Evidence Id
- 19972
- Core Evidence Id
- 19972
- Source Reference Id
- 51
- Herb2 Reference Id
- HBREF000127
- Subject Paper Key
- HERB003288_30305611
- Pubmed Id
- 30305611
- Doi
- 10.1038/s41419-018-1086-8
- Paper Title
- Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis
- Paper Abstract
- Glioma is the most common and malignant form of primary brain tumour, and is characterised by high proliferation and extensive invasion and neurological destruction. Demethylzeylasteral (T-96), which is extracted from Tripterygium wilfordii, is considered to have immunosuppressive, anti-inflammatory and anti-angiogenic effects. Here, the anti-tumour effect of T-96 on glioma was evaluated. Our results demonstrated that T-96 significantly inhibited glioma cell growth and induced cell cycle arrest in G1 phase but did not induce apoptosis. Cell invasion and migration were dramatically suppressed after treatment with T-96. Almost all genes related to cell cycle and DNA replication were downregulated after treatment with T-96. Our results showed that miR-30e-5p was noticeably upregulated after T-96 treatment, and MYBL2, which is involved in cell cycle progression and is a target gene of miR-30e-5p, was significantly reduced in synchrony. Overexpression of MYBL2 partially rescued the T-96-induced inhibition of cell growth and proliferation. Moreover, a miR-30e-5p antagomir significantly reduced the upregulation of miR-30e-5p expression induced by T-96, leading to recovery of MYBL2 expression, and partially rescued the T-96-induced inhibition of cell growth and proliferation. More important, T-96 effectively upregulated miR-30e-5p expression and downregulated MYBL2 expression, thus inhibiting LN-229 cell tumour growth in a mouse model. These results indicated that T-96 might inhibit glioma cell growth by regulating the miR-30e-5p/MYBL2 axis. Our study demonstrated that T-96 might act as a promising agent for malignant glioma therapy.
- Journal
- Cell Death Dis
- Publish Year
- 2018
- Experiment Subject
- mouse; cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Demethylzeylasteral inhibits glioma growth by regulating the miR-30e-5p/MYBL2 axis
- Bilingual Status
- semi_complete