ReferenceID 3210

Shen-Ling-Bai-Zhu-San Improves Dextran Sodium Sulfate-Induced Colitis by Inhibiting Caspase-1/Caspase-11-Mediated Pyroptosis

Front Pharmacol

The traditional Chinese medicine Shen-ling-bai-zhu-san (SLBZS) is described in "Tai Ping Hui Min He Ji Ju Fang." SLBZS has been shown to be effective against many gastrointestinal diseases. The present study aimed to inv

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Reference Id
3210
Evidence Id
19800
Core Evidence Id
19800
Source Reference Id
6395
Herb2 Reference Id
HBREF007192
Subject Paper Key
HBFO000431_32547403
Pubmed Id
32547403
Doi
10.3389/fphar.2020.00814
Paper Title
Shen-Ling-Bai-Zhu-San Improves Dextran Sodium Sulfate-Induced Colitis by Inhibiting Caspase-1/Caspase-11-Mediated Pyroptosis
Paper Abstract
The traditional Chinese medicine Shen-ling-bai-zhu-san (SLBZS) is described in "Tai Ping Hui Min He Ji Ju Fang." SLBZS has been shown to be effective against many gastrointestinal diseases. The present study aimed to investigate the effect of SLBZS on experimental colitis in mice and to define the potential mechanisms. Our data suggest that compared to the model group, SLBZS treatment increases mouse body weight and colon length, decreases the DAI score, and improves colonic injury. SLBZS reduces the production of cytokines (IL-1beta, IL-18, and TNF-alpha) in colon tissue and mouse colonic mucosal epithelial (MCME) cells. Mechanistically, SLBZS inhibits inflammation by inhibiting the MAPK and NF-kappaB signaling pathways. Further mechanistic analyses showed that SLBZS attenuates the expression levels of pyroptosis-related genes, including NLRP3, ASC, and GSDMD-N in the colons of mice. In addition, SLBZS restores the levels of the colon tight junction proteins ZO-1 and occludin, suggesting that it protects colonic barrier integrity and ameliorates the progression of colitis. In this paper, we demonstrate that SLBZS attenuates DSS-induced ulcerative colitis injury in mice via the MAPK/NF-kappaB and pyroptosis signaling pathway. These results indicate that SLBZS is a potential drug for the treatment of UC.
Journal
Front Pharmacol
Publish Year
2020
Experiment Subject
mouse; mouse colonic mucosal epithelial (mcme) cells
Experiment Type
Animal Experiment
Phenotype Related
Colonic Injury; Gastrointestinal Diseases; Colitis; Ulcerative Colitis Injury
Paper Title Cn
Paper Title En
Shen-Ling-Bai-Zhu-San Improves Dextran Sodium Sulfate-Induced Colitis by Inhibiting Caspase-1/Caspase-11-Mediated Pyroptosis
Bilingual Status
semi_complete