ReferenceID 3191

Neuroprotective mechanisms of ε-viniferin in a rotenone-induced cell model of Parkinson's disease: significance of SIRT3-mediated FOXO3 deacetylation

Neural Regen Res

Trans-(-)-epsilon-viniferin (epsilon-viniferin) has antioxidative and anti-inflammatory effects. It also has neuroprotective effects in Huntington's disease by activating the SIRT3/LKB1/AMPK signaling pathway; however, i

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Reference Id
3191
Evidence Id
19781
Core Evidence Id
19781
Source Reference Id
6359
Herb2 Reference Id
HBREF007156
Subject Paper Key
HBIN049233_32394973
Pubmed Id
32394973
Doi
10.4103/1673-5374.282264
Paper Title
Neuroprotective mechanisms of ε-viniferin in a rotenone-induced cell model of Parkinson's disease: significance of SIRT3-mediated FOXO3 deacetylation
Paper Abstract
Trans-(-)-epsilon-viniferin (epsilon-viniferin) has antioxidative and anti-inflammatory effects. It also has neuroprotective effects in Huntington's disease by activating the SIRT3/LKB1/AMPK signaling pathway; however, it remains unknown whether epsilon-viniferin also has a neuroprotective role in Parkinson's disease. A Parkinson's disease cell model was induced by exposing SH-SY5Y cells to 3.0 muM rotenone for 24 hours, and cells were then treated with 1.0 muM epsilon-viniferin for 24 hours. Treatment with epsilon-viniferin upregulated SIRT3 expression, which promoted FOXO3 deacetylation and nuclear localization. epsilon-Viniferin also increased ATP production and decreased reactive oxygen species production. Furthermore, epsilon-viniferin treatment alleviated rotenone-induced mitochondrial depolarization and reduced cell apoptosis, and restored the expression of mitochondrial homeostasis-related proteins. However, when cells were transfected with SIRT3 or FOXO3 shRNA prior to rotenone and epsilon-viniferin treatment, these changes were reversed. The results from the present study indicate that epsilon-viniferin enhances SIRT3-mediated FOXO3 deacetylation, reduces oxidative stress, and maintains mitochondrial homeostasis, thus inhibiting rotenone-induced cell apoptosis. epsilon-Viniferin may therefore be a promising treatment strategy for Parkinson's disease.
Journal
Neural Regen Res
Publish Year
2020
Experiment Subject
Experiment Type
Cell Experiment
Phenotype Related
Parkinson's Disease; Huntington's Disease
Paper Title Cn
Paper Title En
Neuroprotective mechanisms of ε-viniferin in a rotenone-induced cell model of Parkinson's disease: significance of SIRT3-mediated FOXO3 deacetylation
Bilingual Status
semi_complete