ReferenceID 3176

Zearalenone disrupts the placental function of rats: A possible mechanism causing intrauterine growth restriction

Food Chem Toxicol

Zearalenone is an estrogenic mycotoxin produced by a variety of Fusarium fungi. There is evidence that exposure to zearalenone can cause intrauterine growth restriction, but little is known about the mechanism in the rat

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Reference Id
3176
Evidence Id
19766
Core Evidence Id
19766
Source Reference Id
6335
Herb2 Reference Id
HBREF007132
Subject Paper Key
HBIN048859_32858132
Pubmed Id
32858132
Doi
10.1016/j.fct.2020.111698
Paper Title
Zearalenone disrupts the placental function of rats: A possible mechanism causing intrauterine growth restriction
Paper Abstract
Zearalenone is an estrogenic mycotoxin produced by a variety of Fusarium fungi. There is evidence that exposure to zearalenone can cause intrauterine growth restriction, but little is known about the mechanism in the rat placenta caused by zearalenone. From gestational day 14-21, female Sprague Dawley rats (60 days old) were gavaged with zearalenone (0, 2.5, 5, 10, and 20 mg/kg/day body weight). Zearalenone dose-dependently reduced serum LH and FSH levels of dams at >= 5 mg/kg. RNA-seq and qPCR showed that zearalenone significantly down-regulated Slc38a1 expression at 2.5 mg/kg, Echs1 and Pc at 10 mg/kg, as well as Slc1a5, Cd36, Ldlr, Hadhb, and Cyp17a1 expression at a dose of 20 mg/kg, while it up-regulated the expression of Notch signal (Dvl1 and Jag 1). After zearalenone treatment, their proteins showed a similar trend. Zearalenone reduced the phosphorylation of AKT1, ERK1/2, and mTOR at 5 mg/kg or higher and 4EBP1 at 5 mg/kg. Zearalenone also increased BECLIN1, LC3B, and p62 levels and elevated BAX/BCL2 and CASP3/PROCASP3 ratios. In conclusion, zearalenone disrupts placental function such as reduction of nutrient transport and lipid metabolism possibly via AKT1/ERK1/2/mTOR-mediated autophagy and apoptosis.
Journal
Food Chem Toxicol
Publish Year
2020
Experiment Subject
rat; sprague-dawley rat
Experiment Type
Animal Experiment
Phenotype Related
Paper Title Cn
Paper Title En
Zearalenone disrupts the placental function of rats: A possible mechanism causing intrauterine growth restriction
Bilingual Status
semi_complete