ReferenceID 3159
Fengreqing Oral Liquid Exerts Anti-Inflammatory Effects by Promoting Apoptosis and Inhibiting PI3K/AKT and NF-κB Signaling Pathways
Front Pharmacol
Fengreqing oral liquid (FOL), a Chinese patent drug frequently used in clinical practice in China, is effective in treating inflammatory diseases of the upper respiratory tract such as colds and flu. However, its anti-in
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Record Fields
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- Reference Id
- 3159
- Evidence Id
- 19749
- Core Evidence Id
- 19749
- Source Reference Id
- 6466
- Herb2 Reference Id
- HBREF007263
- Subject Paper Key
- HBFO001092_35370749
- Pubmed Id
- 35370749
- Doi
- 10.3389/fphar.2022.824579
- Paper Title
- Fengreqing Oral Liquid Exerts Anti-Inflammatory Effects by Promoting Apoptosis and Inhibiting PI3K/AKT and NF-κB Signaling Pathways
- Paper Abstract
- Fengreqing oral liquid (FOL), a Chinese patent drug frequently used in clinical practice in China, is effective in treating inflammatory diseases of the upper respiratory tract such as colds and flu. However, its anti-inflammatory effects and mechanisms remain to be elucidated. In this study, the anti-inflammatory effects of FOL and its mechanisms on PI3K/AKT and NF-kappaB signaling pathways in LPS-induced RAW264.7 cells were explored, as well as the regulatory effect of FOL on apoptosis. In addition, the potential of FOL for the treatment of acute lung injury was explored in LPS-induced ALI mice. The results showed that treatment with FOL significantly reduced the levels of interleukin 1beta (IL-1beta), interleukin 6 (IL-6), nitric oxide (NO), and tumor necrosis factor alpha (TNF-alpha) in the supernatant of LPS-induced RAW264.7 cells, and also significantly reduced the phosphorylated protein levels of PI3K and AKT in the PI3K/AKT signaling pathway and also protein levels of NF-kappaB p50, phosphorylated NF-kappaB p65, and IkappaBalpha in the NF-kappaB signaling pathway. In addition, the results showed that FOL induced apoptosis in LPS-induced RAW264.7 cells at the level of 80%-90%, and significantly increased the protein expression levels of the pro-apoptotic Bax and cleaved-caspase-3. In LPS-induced ALI mice, FOL administration showed inhibition of IL-1beta, IL-6, and TNF-alpha in Bronchoalveolar lavage fluid (BALF) and decreased protein expression levels of PI3K, AKT, NF-kappaB p50, and NF-kappaB p65, and elevated protein expression levels of Bax and cleaved-caspase-3 significantly. These results suggest that FOL may exert anti-inflammatory effects by inhibiting the PI3K/AKT signaling pathway to promote apoptosis and leading to attenuated activation of the NF-kappaB signaling pathway.
- Journal
- Front Pharmacol
- Publish Year
- 2022
- Experiment Subject
- mouse; raw264.7 cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Tumor; Inflammatory Diseases Of The Upper Respiratory Tract; Acute Lung Injury
- Paper Title Cn
- Paper Title En
- Fengreqing Oral Liquid Exerts Anti-Inflammatory Effects by Promoting Apoptosis and Inhibiting PI3K/AKT and NF-κB Signaling Pathways
- Bilingual Status
- semi_complete