ReferenceID 3140
Vitamin K2 Improves Osteogenic Differentiation by Inhibiting STAT1 via the Bcl-6 and IL-6/JAK in C3H10 T1/2 Clone 8 Cells
Nutrients
Osteogenic activity of vitamin K 2 (VK 2 ), a small molecular nutrient, has been suggested. However, the underlying mechanisms have not been fully elucidated. Therefore, this study aimed to explore the mechanisms by whic
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 3140
- Evidence Id
- 19730
- Core Evidence Id
- 19730
- Source Reference Id
- 6266
- Herb2 Reference Id
- HBREF007063
- Subject Paper Key
- HBIN048069_35889891
- Pubmed Id
- 35889891
- Doi
- 10.3390/nu14142934
- Paper Title
- Vitamin K2 Improves Osteogenic Differentiation by Inhibiting STAT1 via the Bcl-6 and IL-6/JAK in C3H10 T1/2 Clone 8 Cells
- Paper Abstract
- Osteogenic activity of vitamin K 2 (VK 2 ), a small molecular nutrient, has been suggested. However, the underlying mechanisms have not been fully elucidated. Therefore, this study aimed to explore the mechanisms by which VK 2 promotes osteogenic differentiation. The effects of VK 2 on osteogenic differentiation indicators were determined in C3H10 T1/2 clone 8 cells. The RNA-seq analysis was used to explore the hypothesis that VK 2 promotes osteogenic differentiation. Small interfering RNA (siRNA) assay and plasmid transfection assay were used to determine the potential role of VK 2 in the modulation of Bcl-6/STAT axis and IL-6/JAK/STAT signaling pathway. VK 2 significantly increased alkaline phosphatase (ALP) activity, ALP, osteocalcin (OCN), and RUNX2 abundance, and RUNX2 protein expression. RNA-seq analysis showed that there were 314 differentially expressed genes (DEGs) upregulated and 1348 DEGs downregulated by VK 2 . PPI analysis determined the top 10 hub genes upregulated or downregulated by VK 2 . Overexpression of Bcl-6 increased osteogenic differentiation and decreased expression of STAT1. Administration with VK 2 restored the inhibition by siBcl-6 in osteogenic differentiation. Knockdown of IL-6 decreased the mRNA levels of genes associated with the JAK/STAT signaling pathway, and increased markers of osteoblast differentiation. Furthermore, treatment with VK 2 improved inhibition in osteogenic differentiation and decreased enhancement of JAK/STAT signaling pathway related genes by overexpression of IL-6. Our study suggests that VK 2 could improve osteogenic differentiation via the Bcl-6/STAT axis and IL-6/JAK/STAT signaling pathway.
- Journal
- Nutrients
- Publish Year
- 2022
- Experiment Subject
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Vitamin K2 Improves Osteogenic Differentiation by Inhibiting STAT1 via the Bcl-6 and IL-6/JAK in C3H10 T1/2 Clone 8 Cells
- Bilingual Status
- semi_complete