ReferenceID 3128
Vitamin B6 inhibits macrophage activation to prevent lipopolysaccharide-induced acute pneumonia in mice
J Cell Mol Med
Macrophage activation participates in the pathogenesis of pulmonary inflammation. As a coenzyme, vitamin B6 (VitB6) is mainly involved in the metabolism of amino acids, nucleic acids, glycogen and lipids. We have previou
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Record Fields
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- Reference Id
- 3128
- Evidence Id
- 19718
- Core Evidence Id
- 19718
- Source Reference Id
- 6252
- Herb2 Reference Id
- HBREF007049
- Subject Paper Key
- HBIN048045_31970902
- Pubmed Id
- 31970902
- Doi
- 10.1111/jcmm.14983
- Paper Title
- Vitamin B6 inhibits macrophage activation to prevent lipopolysaccharide-induced acute pneumonia in mice
- Paper Abstract
- Macrophage activation participates in the pathogenesis of pulmonary inflammation. As a coenzyme, vitamin B6 (VitB6) is mainly involved in the metabolism of amino acids, nucleic acids, glycogen and lipids. We have previously reported that activation of AMP-activated protein kinase (AMPK) produces anti-inflammatory effects both in vitro and in vivo. Whether VitB6 via AMPK activation prevents pulmonary inflammation remains unknown. The model of acute pneumonia was induced by injecting mice with lipopolysaccharide (LPS). The inflammation was determined by measuring the levels of interleukin-1 beta (IL-1beta), IL-6 and tumour necrosis factor alpha (TNF-alpha) using real time PCR, ELISA and immunohistochemistry. Exposure of cultured primary macrophages to VitB6 increased AMP-activated protein kinase (AMPK) Thr172 phosphorylation in a time/dose-dependent manner, which was inhibited by compound C. VitB6 downregulated the inflammatory gene expressions including IL-1beta, IL-6 and TNF-alpha in macrophages challenged with LPS. These effects of VitB6 were mirrored by AMPK activator 5-aminoimidazole-4-carboxamide ribonucleoside (AICAR). However, VitB6 was unable to inhibit LPS-induced macrophage activation if AMPK was in deficient through siRNA-mediated approaches. Further, the anti-inflammatory effects produced by VitB6 or AICAR in LPS-treated macrophages were abolished in DOK3 gene knockout (DOK3-/- ) macrophages, but were enhanced in macrophages if DOK3 was overexpressed. In vivo studies indicated that administration of VitB6 remarkably inhibited LPS-induced both systemic inflammation and acute pneumonia in wild-type mice, but not in DOK3-/- mice. VitB6 prevents LPS-induced acute pulmonary inflammation in mice via the inhibition of macrophage activation.
- Journal
- J Cell Mol Med
- Publish Year
- 2020
- Experiment Subject
- mouse; cultured primary macrophages; dok3 gene knockout (dok3-/- ) macrophages; lps-treated macrophages
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Pulmonary Inflammation; Systemic Inflammation; Acute Pneumonia; Acute Pulmonary Inflammation
- Paper Title Cn
- Paper Title En
- Vitamin B6 inhibits macrophage activation to prevent lipopolysaccharide-induced acute pneumonia in mice
- Bilingual Status
- semi_complete