ReferenceID 3114
Anti-inflammatory properties of uvaol on DSS-induced colitis and LPS-stimulated macrophages
Chin Med
Background: Apocynum venetum leaves are used as a kind of phytomedicine and the main ingredient in some traditional Chinese medicine products for the relief of colitis. To understand the bioactive constituents of A. vene
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Record Fields
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- Reference Id
- 3114
- Evidence Id
- 19704
- Core Evidence Id
- 19704
- Source Reference Id
- 6219
- Herb2 Reference Id
- HBREF007016
- Subject Paper Key
- HBIN047656_32411289
- Pubmed Id
- 32411289
- Doi
- 10.1186/s13020-020-00322-0
- Paper Title
- Anti-inflammatory properties of uvaol on DSS-induced colitis and LPS-stimulated macrophages
- Paper Abstract
- Background: Apocynum venetum leaves are used as a kind of phytomedicine and the main ingredient in some traditional Chinese medicine products for the relief of colitis. To understand the bioactive constituents of A. venetum L., we did a phytochemistry study and investigated anti-Inflammatory effects of compounds and explored the underlying mechanisms. Methods: We isolated compounds from ethanol extract of A. venetum L. leaf and detected the most effective compound by NO inhibition assay. We investigated anti-Inflammatory effects on dextran sulfate sodium (DSS)-induced colitis mice and lipopolysaccharide (LPS)-stimulated RAW264.7 cells. The disease activity index was determined by scores of body weight loss, diarrhea and rectal bleeding; histological damage was analyzed by H&E staining; macrophages change in the colon were analyzed by immunohistochemistry (IHC); myeloperoxidase activity was measured by myeloperoxidase assay kits; levels of proinflammatory cytokines were determined by qPCR and ELISA; protein production such as COX-2, iNOS, STAT3 and ERK1/2 were determined by western blotting. Results: We isolated uvaol from ethanol extract of A. venetum L. leaf and found uvaol has excellent potential of inhibiting NO production. We further found uvaol could attenuate disease activity index (DAI), colon shortening, colon injury, and colonic myeloperoxidase activity in DSS-induced colitis mice. Moreover, uvaol significantly reduces mRNA expression and production of pro-inflammatory cytokines (TNF-alpha, IL-6, IL-1beta, and MCP-1) and infiltration of macrophages in colonic tissues of colitis mice. Studies on LPS challenged murine macrophage RAW246.7 cells also revealed that uvaol reduces mRNA expression and production of pro-inflammatory cytokines and mediators. Mechanically, uvaol inhibits the pro-inflammatory ERK/STAT3 axis in both inflamed colonic tissues and macrophages. Conclusions: A. venetum leaf contains uvaol and uvaol has potent anti-inflammatory effects on DSS-induced experimental colitis and LPS-stimulated RAW264.7 macrophage cells. These results suggest uvaol is a prospective anti-inflammatory agent for colonic inflammation.
- Journal
- Chin Med
- Publish Year
- 2020
- Experiment Subject
- mouse; lipopolysaccharide (lps)-stimulated raw264.7 cells; lps-stimulated raw264.7 macrophage cells; murine macrophage raw246.7 cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Rectal Bleeding; Colonic Inflammation; Colon Injury; Colitis; Diarrhea
- Paper Title Cn
- Paper Title En
- Anti-inflammatory properties of uvaol on DSS-induced colitis and LPS-stimulated macrophages
- Bilingual Status
- semi_complete