ReferenceID 3095
Ursodeoxycholic acid attenuates the expression of proinflammatory cytokines in periodontal cells
J Periodontol
BACKGROUND: Ursodeoxycholic acid (UDCA) is one of the first-line therapeutic medications used in treatment of cholestatic liver disease. Considering that periodontitis is epidemiologically linked to liver diseases, the q
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Record Fields
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- Reference Id
- 3095
- Evidence Id
- 19685
- Core Evidence Id
- 19685
- Source Reference Id
- 6186
- Herb2 Reference Id
- HBREF006983
- Subject Paper Key
- HBIN047610_31960968
- Pubmed Id
- 31960968
- Doi
- 10.1002/JPER.19-0013
- Paper Title
- Ursodeoxycholic acid attenuates the expression of proinflammatory cytokines in periodontal cells
- Paper Abstract
- BACKGROUND: Ursodeoxycholic acid (UDCA) is one of the first-line therapeutic medications used in treatment of cholestatic liver disease. Considering that periodontitis is epidemiologically linked to liver diseases, the question arises weather UDCA holds anti-inflammatory properties on periodontal health. Herein, we provide information that support anti-inflammatory effects of UDCA on three different periodontium-related cell types. METHODS: Gingival fibroblasts and the oral human squamous carcinoma cell line HSC-2 were exposed to interleukin (IL)1beta and tumor necrosis factor (TNF)alpha with and without UDCA. Murine RAW 264.7 macrophages were incubated with sterile-filtered human saliva also in the presence of UDCA. The expression of inflammatory cytokines was measured by reverse transcription-polymerase chain reaction. Immunoassay was applied to detect the production of IL6. Immunostaining was performed for the p65 subunit to further support the anti-inflammatory role of UDCA. RESULTS: We report here that UDCA significantly reduced the IL1beta and TNFalpha-induced expression of IL1, IL6, and IL8 in gingival fibroblasts and the HSC-2 cell line. In RAW 264.7 macrophages, UDCA attenuated the expression of IL1alpha, IL1beta, and IL6 that was increased by saliva. Immunoassay confirmed the capacity of UDCA to reduce inflammation-induced production of IL6 in gingival fibroblasts, HSC-2 and RAW 264.7 cells. Immunostaining revealed the blocking of nuclear translocation of p65 in gingival fibroblasts. CONCLUSIONS: Taken together, UDCA can attenuate the provoked expression of inflammatory cytokines in oral fibroblasts, oral human squamous carcinoma cells and macrophages in vitro. These data support the hypothesis that patients with cholestatic liver disease might benefit from UDCA with respect to periodontal health.
- Journal
- J Periodontol
- Publish Year
- 2020
- Experiment Subject
- mouse; human; patient; hsc-2 cell line; murine raw 264.7 macrophages; oral human squamous carcinoma cell line hsc-2; raw 264.7 cells; raw 264.7 macrophages
- Experiment Type
- Cell Experiment
- Phenotype Related
- Squamous Carcinoma; Tumor; Liver Diseases; Cholestatic Liver Disease; Periodontitis
- Paper Title Cn
- Paper Title En
- Ursodeoxycholic acid attenuates the expression of proinflammatory cytokines in periodontal cells
- Bilingual Status
- semi_complete