ReferenceID 2984
Theobromine alleviates diet-induced obesity in mice via phosphodiesterase-4 inhibition
Eur J Nutr
PURPOSE: Modern science has given much attention to the treatment of obesity by activating brown adipose tissue (BAT) and browning of white adipose tissue (WAT). Recent studies have identified theobromine, a derivative o
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Record Fields
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- Reference Id
- 2984
- Evidence Id
- 19574
- Core Evidence Id
- 19574
- Source Reference Id
- 5968
- Herb2 Reference Id
- HBREF006765
- Subject Paper Key
- HBIN046280_31965293
- Pubmed Id
- 31965293
- Doi
- 10.1007/s00394-020-02184-6
- Paper Title
- Theobromine alleviates diet-induced obesity in mice via phosphodiesterase-4 inhibition
- Paper Abstract
- PURPOSE: Modern science has given much attention to the treatment of obesity by activating brown adipose tissue (BAT) and browning of white adipose tissue (WAT). Recent studies have identified theobromine, a derivative of cocoa, as a potent natural component actively browning white fat cells. Here, we aimed to deduce the anti-obesity effect of theobromine involving phosphodiesterase (PDE) dependent-regulatory pathway in obese animal models. METHODS: For examining activity of theobromine, C57BL/6 mice were fed with high fat diet and treated with theobromine to determine the expression levels of protein markers by immunoblot analysis and gene targets by quantitative real-time PCR. Other methods used include histopathological studies, immunofluorescence and molecular docking approaches. RESULTS: Theobromine alleviated diet-induced obesity in mice by browning of iWAT and activating BAT. Further, theobromine actively interacted with PDE4D and inhibited its activity in adipose tissues and cells potentiating energy expenditure. Moreover, the regulatory action of theobromine via inhibition of PDE4D was mediated by beta3-AR signaling pathway. CONCLUSION: Altogether, the current results signifies critical role of theobromine in reducing obesity by regulation of lipid metabolism through inhibition of PDE4, indicating its potential as a major therapeutic medicinal compound.
- Journal
- Eur J Nutr
- Publish Year
- 2020
- Experiment Subject
- mouse
- Experiment Type
- Animal Experiment
- Phenotype Related
- Obesity; Obese
- Paper Title Cn
- Paper Title En
- Theobromine alleviates diet-induced obesity in mice via phosphodiesterase-4 inhibition
- Bilingual Status
- semi_complete