ReferenceID 2888
Study on alleviation effect of stachyose on food allergy through TLR2/NF-κB signal pathway in a mouse model
Life Sci
AIMS: To explore the effect on food allergy of stachyose. MAIN METHODS: The egg allergen ovalbumin (OVA) was used to induce a food allergy model of BALB/c mice, and different doses of stachyose were given in process. Usi
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Record Fields
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- Reference Id
- 2888
- Evidence Id
- 19478
- Core Evidence Id
- 19478
- Source Reference Id
- 5762
- Herb2 Reference Id
- HBREF006559
- Subject Paper Key
- HBIN044691_34653427
- Pubmed Id
- 34653427
- Doi
- 10.1016/j.lfs.2021.120038
- Paper Title
- Study on alleviation effect of stachyose on food allergy through TLR2/NF-κB signal pathway in a mouse model
- Paper Abstract
- AIMS: To explore the effect on food allergy of stachyose. MAIN METHODS: The egg allergen ovalbumin (OVA) was used to induce a food allergy model of BALB/c mice, and different doses of stachyose were given in process. Using enzyme-linked immunosorbent assay (ELISA) methods to detect the levels of IgE, IgG1, histamine and cytokines. And flow cytometry was used to analyze TH1/TH2 balance further. Besides, Hematoxylin-eosin (HE) staining was used to observe changes of intestinal morphology. Lastly, Reverse Transcription-Polymerase Chain Reaction (RT-qPCR) and western Blot was conducted to explore the possible mechanism. KEY FINDINGS: Compared with OVA group, serum IgE and IgG1 levels in the low-dose (1mg/mouse) group and high-dose (5mg/mouse) group of stachyose were significantly reduced (P < 0.05); the level of plasma histamine was also decreased significantly (P < 0.05) and the body temperature were decreased. In all, allergic symptoms were alleviated after stachyose treatment. Furthermore, TH1/TH2 balance was improved after stachyose treatment. Lastly, the expression of TLR2 and NF-kappaB were increased significantly (P < 0.05) in both mRNA and protein levels after stachyose treatment. SIGNIFICANCE: Food allergy was alleviated through improving TH1/TH2 balance by activating TLR2/NF-kappaB signal by stachyose.
- Journal
- Life Sci
- Publish Year
- 2021
- Experiment Subject
- mouse
- Experiment Type
- Animal Experiment
- Phenotype Related
- Stachyose
- Paper Title Cn
- Paper Title En
- Study on alleviation effect of stachyose on food allergy through TLR2/NF-κB signal pathway in a mouse model
- Bilingual Status
- semi_complete