ReferenceID 2886
Stachydrine attenuates IL-1β-induced inflammatory response in osteoarthritis chondrocytes through the NF-κB signaling pathway
Chem Biol Interact
Osteoarthritis (OA) is a common degenerative joint disease that is closely associated with inflammation. Stachydrine (STA) is a bioactive alkaloid with anti-inflammatory activity. However, the role of STA in OA remains u
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Record Fields
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- Reference Id
- 2886
- Evidence Id
- 19476
- Core Evidence Id
- 19476
- Source Reference Id
- 5759
- Herb2 Reference Id
- HBREF006556
- Subject Paper Key
- HBIN044690_32417162
- Pubmed Id
- 32417162
- Doi
- 10.1016/j.cbi.2020.109136
- Paper Title
- Stachydrine attenuates IL-1β-induced inflammatory response in osteoarthritis chondrocytes through the NF-κB signaling pathway
- Paper Abstract
- Osteoarthritis (OA) is a common degenerative joint disease that is closely associated with inflammation. Stachydrine (STA) is a bioactive alkaloid with anti-inflammatory activity. However, the role of STA in OA remains unknown. This study aimed to explore the effects of STA on OA chondrocytes in the presence of IL-1beta. Primary human OA chondrocytes were pretreated with various concentrations of STA for 2 h and then stimulated with IL-1beta for 24 h. Inflammatory mediators and cytokines including NO, PGE2, TNF-alpha and IL-6 in chondrocytes were detected to reflect inflammation status. Production of extracellular matrix (ECM) degrading enzymes including MMP-3, MMP-13, ADAMTS-4 and ADAMTS-5 in chondrocytes was measured using ELISA. The expression levels of iNOS, COX-2, p65, p-p65, p-IkappaBalpha, and IkappaBalpha were detected by Western blot analysis. Our results showed that STA significantly suppressed IL-1beta-induced inflammation with decreased levels of inflammatory mediators and cytokines including NO, PGE2, iNOS, COX-2, TNF-alpha and IL-6. Treatment with STA suppressed the production of ECM degrading enzymes including MMP-3, MMP-13, ADAMTS-4, and ADAMTS-5 in IL-1beta-induced chondrocytes. Furthermore, STA blocked the IL-1beta-mediated potentiation of NF-kappaB pathway in chondrocytes. In conclusion, these findings demonstrated that STA protected chondrocytes from IL-1beta-induced inflammation through the NF-kappaB signaling pathway.
- Journal
- Chem Biol Interact
- Publish Year
- 2020
- Experiment Subject
- human; il-1beta-induced chondrocytes
- Experiment Type
- Cell Experiment
- Phenotype Related
- Inflammation; Degenerative Joint Disease; Osteoarthritis
- Paper Title Cn
- Paper Title En
- Stachydrine attenuates IL-1β-induced inflammatory response in osteoarthritis chondrocytes through the NF-κB signaling pathway
- Bilingual Status
- semi_complete