ReferenceID 2875
Sophoricoside from Styphnolobium japonicum improves experimental atopic dermatitis in mice
Phytomedicine
BACKGROUND: Abnormal immune responses, specifically excessive differentiation of Th2 cells, are associated with the development of atopic dermatitis (AD). Sophoricoside, the genistein-4'-beta-D-glucoside isolated from St
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- Reference Id
- 2875
- Evidence Id
- 19465
- Core Evidence Id
- 19465
- Source Reference Id
- 5739
- Herb2 Reference Id
- HBREF006536
- Subject Paper Key
- HBIN044393_33545490
- Pubmed Id
- 33545490
- Doi
- 10.1016/j.phymed.2021.153463
- Paper Title
- Sophoricoside from Styphnolobium japonicum improves experimental atopic dermatitis in mice
- Paper Abstract
- BACKGROUND: Abnormal immune responses, specifically excessive differentiation of Th2 cells, are associated with the development of atopic dermatitis (AD). Sophoricoside, the genistein-4'-beta-D-glucoside isolated from Styphnolobium japonicum, has previously demonstrated anti-inflammatory and immunosuppressive effects along with IL-3 and IL-5 inhibitory activities. Therefore, we speculated that sophoricoside could regulate AD by regulating abnormal immune responses. PURPOSE: To investigate the role of sophoricoside on AD-like allergic skin inflammation induced by ovalbumin (OVA) or 2,4,6-trinitrochlorobenzene (TNCB) in mouse models. METHODS: Sophoricoside was isolated from the 70% ethanol extract of S. japonicum dried mature seeds. After being submitted to a purification process, its purity was assessed by high-performance liquid chromatography (HPLC). The effects of sophoricoside were determined in vivo by OVA- and TNCB-induced AD-like allergic skin inflammation in mice. Skin tissues were subjected with hematoxylin-eosin (H&E), Giemsa and toluidine blue staining. In vitro CD4+ T cell differentiation was performed and the levels of serum immunoglobulins, cytokines, and genes related to CD4+ T cell differentiation were determined by enzyme-linked immunosorbent assay (ELISA) and quantitative real-time PCR. Cytokine bioassay, mixed lymphocytes reaction and cell viability assay were performed. RESULTS: Topical application of sophoricoside decreased the symptoms of AD-like allergic skin inflammation, including elevated hypertrophic scars with spongiotic epidermis, epidermal hyperplasia, hyperkeratosis, infiltration of immune, and mast cells, dermal thickness, amounts of immunoglobulins, and pro-inflammatory cytokines, and the mast cell population in the skin. Sophoricoside also decreased T cell antigen receptor (TCR)-mediated immune responses. In particular, sophoricoside suppressed the differentiation of naive CD4+ T cells into Th cell subsets, including Th1, Th2, and Th17, by inhibiting the expression of their subset-specific master transcription factors, leading to suppression of the expression and production of these cell subset-specific cytokines. CONCLUSION: Sophoricoside can improve AD-like allergic skin diseases mainly by inhibiting pathogenic CD4+ T cell differentiation and immune responses.
- Journal
- Phytomedicine
- Publish Year
- 2021
- Experiment Subject
- mouse
- Experiment Type
- Cell Experiment
- Phenotype Related
- Ad-like Allergic Skin Diseases; Ad-like Allergic Skin Inflammation; Atopic Dermatitis; Hypertrophic Scars; Hyperkeratosis; Epidermal Hyperplasia; Spongiotic Epidermis
- Paper Title Cn
- Paper Title En
- Sophoricoside from Styphnolobium japonicum improves experimental atopic dermatitis in mice
- Bilingual Status
- semi_complete