ReferenceID 2700
Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters
Virol Sin
The hepatitis B virus (HBV) is a global public health challenge due to its highly contagious nature. It is estimated that almost 300 million people live with chronic HBV infection annually. Although nucleoside analogs ma
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- Reference Id
- 2700
- Evidence Id
- 19290
- Core Evidence Id
- 19290
- Source Reference Id
- 5394
- Herb2 Reference Id
- HBREF006191
- Subject Paper Key
- HBIN041121_35305922
- Pubmed Id
- 35305922
- Doi
- 10.1016/j.virs.2022.01.027
- Paper Title
- Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters
- Paper Abstract
- The hepatitis B virus (HBV) is a global public health challenge due to its highly contagious nature. It is estimated that almost 300 million people live with chronic HBV infection annually. Although nucleoside analogs markedly reduce the risk of liver disease progression, the analogs do not fully eradicate the virus. As such, new treatment options and drugs are urgently needed. Psoralen is a nourishing monomer of Chinese herb and is known to inhibit virus replication and inactivate viruses. In this study, we evaluated the potential of psoralen as an anti-HBV agent. Quantitative PCR and Southern blot analysis revealed that psoralen inhibited HBV replication in HepG2.2.15 cells in a concentration-dependent manner. Moreover, psoralen was also active against the 3TC/ETV-dual-resistant HBV mutant. Further investigations revealed that psoralen suppressed both HBV RNA transcription and core protein expression. The transcription factor FOXO1, a known target for PGC1α co-activation, binds to HBV pre-core/core promoter enhancer II region and activates HBV RNA transcription. Co-immunoprecipitation showed that psoralen suppressed the expression of FOXO1, thereby decreasing the binding of FOXO1 co-activator PGC1α to the HBV promoter. Overall, our results demonstrate that psoralen suppresses HBV RNA transcription by down-regulating the expression of FOXO1 resulting in a reduction of HBV replication.
- Journal
- Virol Sin
- Publish Year
- 2022
- Experiment Subject
- hepg2.2.15 cells; people
- Experiment Type
- Cell Experiment
- Phenotype Related
- Liver Disease; Hepatitis B Virus; Chronic Hbv Infection
- Paper Title Cn
- Paper Title En
- Psoralen inhibits hepatitis B viral replication by down-regulating the host transcriptional machinery of viral promoters
- Bilingual Status
- semi_complete