ReferenceID 2698
Enhancement of skin barrier and hydration-related molecules by protopanaxatriol in human keratinocytes
J Ginseng Res
Background: Protopanaxatriol (PPT) is a secondary intestinal metabolite of ginsenoside in ginseng. Although the effects of PPT have been reported in various diseases including cancer, diabetes and inflammatory diseases,
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Record Fields
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- Reference Id
- 2698
- Evidence Id
- 19288
- Core Evidence Id
- 19288
- Source Reference Id
- 5392
- Herb2 Reference Id
- HBREF006189
- Subject Paper Key
- HBIN040937_33841016
- Pubmed Id
- 33841016
- Doi
- 10.1016/j.jgr.2020.12.003
- Paper Title
- Enhancement of skin barrier and hydration-related molecules by protopanaxatriol in human keratinocytes
- Paper Abstract
- Background: Protopanaxatriol (PPT) is a secondary intestinal metabolite of ginsenoside in ginseng. Although the effects of PPT have been reported in various diseases including cancer, diabetes and inflammatory diseases, the skin protective effects of PPT are poorly understood. Methods: HaCaT cells were treated with PPT in a dose-dependent manner. mRNA and protein levels which related to skin barrier and hydration were detected compared with retinol. Luciferase assay was performed to explore the relative signaling pathway. Western blot was conducted to confirm these pathways and excavated further signals. Results: PPT enhanced the expression of filaggrin (FLG), transglutaminase (TGM)-1, claudin, occludin and hyaluronic acid synthase (HAS) -1, -2 and -3. The mRNA expression levels of FLG, TGM-1, HAS-1 and HAS-2 were suppressed under NF-kappaB inhibition. PPT significantly augmented NF-kappaB-luc activity and upregulated Src/AKT/NF-kappaB signaling. In addition, PPT also increased phosphorylation of the mitogen-activated protein kinases (MAPKs) ERK, JNK and p38 and upstream MAPK activators (MEK and MKK). Furthermore, transcriptional activity of AP-1 and CREB, which are downstream signaling targets of MAPK, was enhanced by PPT. Conclusion: PPT improves skin barrier function and hydration through Src/AKT/NF-kappaB and MAPK signaling. Therefore, PPT may be a valuable component for cosmetics or treating skin disorders.
- Journal
- J Ginseng Res
- Publish Year
- 2021
- Experiment Subject
- Experiment Type
- Cell Experiment
- Phenotype Related
- Diabetes; Skin Disorders; Cancer; Inflammatory Diseases
- Paper Title Cn
- Paper Title En
- Enhancement of skin barrier and hydration-related molecules by protopanaxatriol in human keratinocytes
- Bilingual Status
- semi_complete