ReferenceID 2656
Piceatannol antagonizes lipolysis by promoting autophagy-lysosome-dependent degradation of lipolytic protein clusters in adipocytes
J Nutr Biochem
Overly elevated circulating non-esterified fatty acids (NEFAs) is an emerging health concern of obesity-associated energy disorders. However, methods to reduce circulating NEFAs remain elusive. The present study determin
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 2656
- Evidence Id
- 19246
- Core Evidence Id
- 19246
- Source Reference Id
- 5311
- Herb2 Reference Id
- HBREF006108
- Subject Paper Key
- HBIN040152_35346829
- Pubmed Id
- 35346829
- Doi
- 10.1016/j.jnutbio.2022.108998
- Paper Title
- Piceatannol antagonizes lipolysis by promoting autophagy-lysosome-dependent degradation of lipolytic protein clusters in adipocytes
- Paper Abstract
- Overly elevated circulating non-esterified fatty acids (NEFAs) is an emerging health concern of obesity-associated energy disorders. However, methods to reduce circulating NEFAs remain elusive. The present study determined the effect of piceatannol, a naturally occurring stilbene, on adipocyte lipolysis and its underlying mechanism. Differentiated 3T3-L1 adipocytes, brown adipocytes and isolated white adipose tissue were treated with various concentrations of piceatannol for 1.5-h both in the basal and stimulated lipolysis conditions. Piceatannol significantly inhibited NEFAs and glycerol release with a concomitant reduction of ATGL, CGI-58 and PLIN1 expression in adipocytes. Using a series of inhibitor assays, piceatannol-induced degradation of these proteins was found to be mediated by upregulation of the autophagy-lysosome pathway. Moreover, we demonstrated that piceatannol is capable of stimulating autophagy in vitro. Importantly, piceatannol administration tended to lower fasting-induced serum glycerol levels in healthy mice. Furthermore, piceatannol administration lowered lipolysis, central adiposity and hyperinsulinemia in diet-induced obese mice. Our study provides profound evidence of a novel inhibitory role of piceatannol in lipolysis through autophagy-lysosome-dependent degradation of the key lipolytic proteins in adipocytes. This study offers a mechanistic foundation for investigating the potential of piceatannol-containing foods in reducing lipolysis and its associated metabolic disorders.
- Journal
- J Nutr Biochem
- Publish Year
- 2022
- Experiment Subject
- mouse; differentiated 3t3-l1 adipocytes
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Metabolic Disorders; Obesity-associated Energy Disorders; Obese; Hyperinsulinemia
- Paper Title Cn
- Paper Title En
- Piceatannol antagonizes lipolysis by promoting autophagy-lysosome-dependent degradation of lipolytic protein clusters in adipocytes
- Bilingual Status
- semi_complete