ReferenceID 2605
Phloretin Protects Bovine Rumen Epithelial Cells from LPS-Induced Injury
Toxins (Basel)
Lipopolysaccharide (LPS) is an endotoxin that induces immune and inflammatory responses in the rumen epithelium of dairy cows. It is well-known that flavonoid phloretin (PT) exhibits anti-oxidative, anti-inflammatory and
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Record Fields
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- Reference Id
- 2605
- Evidence Id
- 19195
- Core Evidence Id
- 19195
- Source Reference Id
- 5211
- Herb2 Reference Id
- HBREF006008
- Subject Paper Key
- HBIN039576_35622584
- Pubmed Id
- 35622584
- Doi
- 10.3390/toxins14050337
- Paper Title
- Phloretin Protects Bovine Rumen Epithelial Cells from LPS-Induced Injury
- Paper Abstract
- Lipopolysaccharide (LPS) is an endotoxin that induces immune and inflammatory responses in the rumen epithelium of dairy cows. It is well-known that flavonoid phloretin (PT) exhibits anti-oxidative, anti-inflammatory and antibacterial activity. The aim of this research was to explore whether PT could decrease LPS-induced damage to bovine rumen epithelial cells (BRECs) and its molecular mechanisms of potential protective efficacy. BRECs were pretreated with PT for 2 h and then stimulated with LPS for the assessment of various response indicators. The results showed that 100 µM PT had no significant effect on the viability of 10 µg/mL LPS-induced BRECs, and this dose was used in follow-up studies. The results showed that PT pre-relieved the decline in LPS-induced antioxidant indicators (T-AOC and GSH-PX). PT pretreatment resulted in decreased interleukin-1β (IL-1β), IL-6, IL-8, tumor necrosis factor-α (TNF-α) and chemokines (CCL2, CCL5, CCL20) expression. The underlying mechanisms explored reveal that PT may contribute to inflammatory responses by regulating Toll-like receptor 4 (TLR4), nuclear transcription factor-κB p65 (NF-κB p65), and ERK1/2 (p42/44) signaling pathways. Moreover, further studies found that LPS-induced BRECs showed decreased expression of claudin-related genes (ZO-1, Occludin); these were attenuated by pretreatment with PT. These results suggest that PT enhances the antioxidant properties of BRECs during inflammation, reduces gene expression of pro-inflammatory cytokines and chemokines, and enhances barrier function. Overall, the results suggest that PT (at least in vitro) offers some protective effect against LPS-induced ruminal epithelial inflammation. Further in vivo studies should be conducted to identify strategies for the prevention and amelioration of short acute rumen acidosis (SARA) in dairy cows using PT.
- Journal
- Toxins (Basel)
- Publish Year
- 2022
- Experiment Subject
- bovine; cow
- Experiment Type
- Cell Experiment
- Phenotype Related
- Short Acute Rumen Acidosis; Tumor
- Paper Title Cn
- Paper Title En
- Phloretin Protects Bovine Rumen Epithelial Cells from LPS-Induced Injury
- Bilingual Status
- semi_complete