ReferenceID 2548
Osthole Attenuates Macrophage Activation in Experimental Asthma by Inhibitingthe NF-ĸB/MIF Signaling Pathway
Front Pharmacol
Inhibition of activated macrophages is an alternative therapeutic strategy for asthma. We investigated whether a coumarin compound, osthole, isolated from Cnidium monnieri (L.) Cuss, alleviated macrophage activation in v
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Record Fields
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- Reference Id
- 2548
- Evidence Id
- 19138
- Core Evidence Id
- 19138
- Source Reference Id
- 5102
- Herb2 Reference Id
- HBREF005899
- Subject Paper Key
- HBIN038387_33828480
- Pubmed Id
- 33828480
- Doi
- 10.3389/fphar.2021.572463
- Paper Title
- Osthole Attenuates Macrophage Activation in Experimental Asthma by Inhibitingthe NF-ĸB/MIF Signaling Pathway
- Paper Abstract
- Inhibition of activated macrophages is an alternative therapeutic strategy for asthma. We investigated whether a coumarin compound, osthole, isolated from Cnidium monnieri (L.) Cuss, alleviated macrophage activation in vivo and in vitro. Osthole could reduce expression of a marker of activated macrophages, cluster of differentiation (CD)206, in an ovalbumin-challenge model of asthma in mice. Osthole could also inhibit infiltration of inflammatory cells, collagen deposition and production of proinflammatory cytokines [interleukin (IL)-1beta, tumor necrosis factor-alpha, macrophage migration inhibitory factor (MIF)] in asthmatic mice. In vitro, expression of phosphorylated-IkBalpha, MIF and M2 cytokines (Ym-1, Fizz-1, arginase-1) in IL-4-induced macrophages decreased upon exposure to the NF-kB inhibitor MG-132. In our short hairpin (sh)RNA-MIF-knockdown model, reduced expression of M2 cytokines was detected in the IL-4 + shRNA-MIF group. Osthole could attenuate the proliferation and migration of an IL-4-induced rat alveolar macrophages line (NR8383). Osthole could reduce IL-4-induced translocation of nuclear factor-kappa B (NF-kB) in NR8383 cells. Collectively, our results suggest that osthole ameliorates macrophage activation in asthma by suppressing the NF-kB/MIF signaling pathway, and might be a potential agent for treating asthma.
- Journal
- Front Pharmacol
- Publish Year
- 2021
- Experiment Subject
- mouse; rat; il-4-induced rat alveolar macrophages line; nr8383 cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Asthma
- Paper Title Cn
- Paper Title En
- Osthole Attenuates Macrophage Activation in Experimental Asthma by Inhibitingthe NF-ĸB/MIF Signaling Pathway
- Bilingual Status
- semi_complete