ReferenceID 2528
Ophiopogonin D promotes bone regeneration by stimulating CD31hi EMCNhi vessel formation
Cell Prolif
OBJECTIVES: CD31hi EMCNhi vessels (CD31, also known as PECAM1 [platelet and endothelial cell adhesion molecule 1]; EMCN, endomucin), which are strongly positive for CD31 and endomucin, couple angiogenesis and osteogenesi
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Record Fields
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- Reference Id
- 2528
- Evidence Id
- 19118
- Core Evidence Id
- 19118
- Source Reference Id
- 5062
- Herb2 Reference Id
- HBREF005859
- Subject Paper Key
- HBIN038205_32080957
- Pubmed Id
- 32080957
- Doi
- 10.1111/cpr.12784
- Paper Title
- Ophiopogonin D promotes bone regeneration by stimulating CD31hi EMCNhi vessel formation
- Paper Abstract
- OBJECTIVES: CD31hi EMCNhi vessels (CD31, also known as PECAM1 [platelet and endothelial cell adhesion molecule 1]; EMCN, endomucin), which are strongly positive for CD31 and endomucin, couple angiogenesis and osteogenesis. However, the role of CD31hi EMCNhi vessels in bone regeneration remains unknown. In the present study, we investigated the role of CD31hi EMCNhi vessels in the process of bone regeneration. MATERIALS AND METHODS: We used endothelial-specific Kruppel like factor 3 (Klf3) knockout mice and ophiopogonin D treatment to interfere with CD31hi EMCNhi vessel formation. We constructed a bone regeneration model by surgical ablation of the trabecular bone. Immunofluorescence and micro-computed tomography (CT) were used to detect CD31hi EMCNhi vessels and bone formation. RESULTS: CD31hi EMCNhi vessels participate in the process of bone regeneration, such that endothelial-specific Klf3 knockout mice showed increased CD31hi EMCNhi vessels and osteoprogenitors in the bone regeneration area, and further accelerated bone formation. We also demonstrated that the natural compound, ophiopogonin D, acts as a KLF3 inhibitor to promote vessels formation both in vitro and in vivo. Administration of ophiopogonin D increased the abundance of CD31hi Emcnhi vessels and accelerated bone healing. CONCLUSIONS: Our findings confirmed the important role of CD31hi Emcnhi vessels in bone regeneration and provided a new target to treat bone fracture or promote bone regeneration.
- Journal
- Cell Prolif
- Publish Year
- 2020
- Experiment Subject
- mouse
- Experiment Type
- Animal Experiment
- Phenotype Related
- Bone Fracture; Osteogenesis
- Paper Title Cn
- Paper Title En
- Ophiopogonin D promotes bone regeneration by stimulating CD31hi EMCNhi vessel formation
- Bilingual Status
- semi_complete