ReferenceID 2479

Nepetin inhibits osteoclastogenesis by inhibiting RANKL-induced activation of NF-κB and MAPK signalling pathway, and autophagy

J Cell Mol Med

Aseptic prosthetic loosening due to wear particle-induced inflammatory osteolysis is the main cause of failure for artificial joint replacement. The inflammatory response and the production of pro-osteoclastic factors le

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Reference Id
2479
Evidence Id
19069
Core Evidence Id
19069
Source Reference Id
4981
Herb2 Reference Id
HBREF005778
Subject Paper Key
HBIN036777_33135301
Pubmed Id
33135301
Doi
10.1111/jcmm.16055
Paper Title
Nepetin inhibits osteoclastogenesis by inhibiting RANKL-induced activation of NF-κB and MAPK signalling pathway, and autophagy
Paper Abstract
Aseptic prosthetic loosening due to wear particle-induced inflammatory osteolysis is the main cause of failure for artificial joint replacement. The inflammatory response and the production of pro-osteoclastic factors lead to elevation of osteoclast formation and excessive activity results in extensive bone destruction around the bone-implant interface. Here we showed that Nepetin, a natural bioactive flavonoid with proven anti-inflammatory and anti-proliferative properties, potently inhibited RANKL-induced osteoclast differentiation, formation and bone resorption in vitro, and protected mice against the deleterious effects of titanium particle-induced calvarial osteolysis in vivo. Mechanistically, Nepetin attenuated RANKL-induced activation of NF-kappaB and MAPK signalling pathways and TRAF6-dependent ubiquitination of Beclin 1 which is necessary for the induction of autophagy. In brief, our study demonstrates the potential therapeutic application of Nepetin against osteoclast-mediated osteolytic diseases.
Journal
J Cell Mol Med
Publish Year
2020
Experiment Subject
mouse
Experiment Type
Animal Experiment
Phenotype Related
Osteolytic Diseases; Calvarial Osteolysis; Inflammatory Osteolysis; Failure For Artificial Joint Replacement; Aseptic Prosthetic Loosening
Paper Title Cn
Paper Title En
Nepetin inhibits osteoclastogenesis by inhibiting RANKL-induced activation of NF-κB and MAPK signalling pathway, and autophagy
Bilingual Status
semi_complete