ReferenceID 2440

Myo-Inositol Supplementation Alleviates Cisplatin-Induced Acute Kidney Injury via Inhibition of Ferroptosis

Cells

Myo -inositol, a carbocyclic sugar, is believed to be relevant to renal pathobiology since the kidney is the major site for its catabolism. Its role in acute kidney injury (AKI) has not been fully investigated. Ferroptos

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Reference Id
2440
Evidence Id
19030
Core Evidence Id
19030
Source Reference Id
4886
Herb2 Reference Id
HBREF005683
Subject Paper Key
HBIN036059_36611810
Pubmed Id
36611810
Doi
10.3390/cells12010016
Paper Title
Myo-Inositol Supplementation Alleviates Cisplatin-Induced Acute Kidney Injury via Inhibition of Ferroptosis
Paper Abstract
Myo -inositol, a carbocyclic sugar, is believed to be relevant to renal pathobiology since the kidney is the major site for its catabolism. Its role in acute kidney injury (AKI) has not been fully investigated. Ferroptosis, a unique form of regulated cell death, is involved in various types of renal injuries. The relevance of myo -inositol with respect to the process of ferroptosis has not been explored either. Herein, our current exploratory studies revealed that supplementation of myo -inositol attenuates cisplatin-induced injury in cultured Boston University mouse proximal tubular (BUMPT) cells and renal tubules in vivo. Moreover, our studies unraveled that metabolic parameters pertaining to ferroptosis were disrupted in cisplatin-treated proximal tubular cells, which were seemingly remedied by the administration of myo -inositol. Mechanistically, we noted that cisplatin treatment led to the up-regulation of NOX4, a key enzyme relevant to ferroptosis, which was normalized by the administration of myo -inositol. Furthermore, we observed that changes in the NOX4 expression induced by cisplatin or myo -inositol were modulated by carboxy-terminus of Hsc70-interacting protein (CHIP), an E3 ubiquitin ligase. Taken together, our investigation suggests that myo -inositol promotes CHIP-mediated ubiquitination of NOX4 to decelerate the process of ferroptosis, leading to the amelioration of cisplatin-induced AKI.
Journal
Cells
Publish Year
2023
Experiment Subject
mouse; cisplatin-treated proximal tubular cells; cultured boston university mouse proximal tubular (bumpt) cells
Experiment Type
Cell Experiment
Phenotype Related
Cisplatin-; Renal Injuries; Ferroptosis; Acute Kidney Injury
Paper Title Cn
Paper Title En
Myo-Inositol Supplementation Alleviates Cisplatin-Induced Acute Kidney Injury via Inhibition of Ferroptosis
Bilingual Status
semi_complete