ReferenceID 2410
Methylglyoxal impairs ATP- and UTP-induced relaxation in the rat carotid arteries
Eur J Pharmacol
Although methylglyoxal (MGO), a highly reactive dicarbonyl compound, influences the functioning of the vasculature, modulating its effects on vascular reactivity to various substances remains unclear, especially purinoce
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Record Fields
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- Reference Id
- 2410
- Evidence Id
- 19000
- Core Evidence Id
- 19000
- Source Reference Id
- 4840
- Herb2 Reference Id
- HBREF005637
- Subject Paper Key
- HBIN035246_36113554
- Pubmed Id
- 36113554
- Doi
- 10.1016/j.ejphar.2022.175259
- Paper Title
- Methylglyoxal impairs ATP- and UTP-induced relaxation in the rat carotid arteries
- Paper Abstract
- Although methylglyoxal (MGO), a highly reactive dicarbonyl compound, influences the functioning of the vasculature, modulating its effects on vascular reactivity to various substances remains unclear, especially purinoceptor ligands. Therefore, we sought to investigate the direct effects of MGO on relaxation induced by adenosine 5'-triphosphate (ATP) and uridine 5'-triphosphate (UTP) in isolated rat carotid arteries. When carotid arteries were exposed to MGO (420 μM for 1 h), relaxation induced by acetylcholine or sodium nitroprusside was not affected by MGO. However, ATP- and UTP-induced relaxation was impaired by MGO compared with the control. In both ATP- and UTP-induced relaxation, endothelial denudation, incubation with the nitric oxide (NO) synthase inhibitor N G -nitro-L-arginine or the selective P2Y purinoceptor 2 (P2Y 2 ) receptor antagonist AR-C118925XX reduced relaxation in both the control and MGO groups, while the differences between the control and MGO groups were eliminated. The cyclooxygenase (COX) inhibitor indomethacin inhibited the differences in ATP/UTP-mediated relaxations between the control and MGO groups. Moreover, N-acetyl-L-cysteine (NAC), an antioxidant, could augment carotid arterial relaxation induced by ATP/UTP in the presence of MGO. MGO increased arachidonic acid-induced contraction, which was suppressed by NAC. Following both ATP/UTP stimulation, MGO increased the release of prostanoids. These results suggest that MGO impaired ATP- and UTP-induced relaxation in carotid arteries, which was caused by suppressed P2Y 2 receptor-mediated signaling and reductions in endothelial NO. Moreover, MGO partially contributed to COX-derived vasoconstrictor prostanoids through increased oxidative stress.
- Journal
- Eur J Pharmacol
- Publish Year
- 2022
- Experiment Subject
- rat
- Experiment Type
- Animal Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- Methylglyoxal impairs ATP- and UTP-induced relaxation in the rat carotid arteries
- Bilingual Status
- semi_complete