ReferenceID 2298

Phagosomal removal of fungal melanin reprograms macrophage metabolism to promote antifungal immunity

Nat Commun

In response to infection, macrophages adapt their metabolism rapidly to enhance glycolysis and fuel specialized antimicrobial effector functions. Here we show that fungal melanin is an essential molecule required for the

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Reference Id
2298
Evidence Id
18888
Core Evidence Id
18888
Source Reference Id
4625
Herb2 Reference Id
HBREF005422
Subject Paper Key
HBIN034656_32385235
Pubmed Id
32385235
Doi
10.1038/s41467-020-16120-z
Paper Title
Phagosomal removal of fungal melanin reprograms macrophage metabolism to promote antifungal immunity
Paper Abstract
In response to infection, macrophages adapt their metabolism rapidly to enhance glycolysis and fuel specialized antimicrobial effector functions. Here we show that fungal melanin is an essential molecule required for the metabolic rewiring of macrophages during infection with the fungal pathogen Aspergillus fumigatus. Using pharmacological and genetic tools, we reveal a molecular link between calcium sequestration by melanin inside the phagosome and induction of glycolysis required for efficient innate immune responses. By remodeling the intracellular calcium machinery and impairing signaling via calmodulin, melanin drives an immunometabolic signaling axis towards glycolysis with activation of hypoxia-inducible factor 1 subunit alpha (HIF-1alpha) and phagosomal recruitment of mammalian target of rapamycin (mTOR). These data demonstrate a pivotal mechanism in the immunometabolic regulation of macrophages during fungal infection and highlight the metabolic repurposing of immune cells as a potential therapeutic strategy.
Journal
Nat Commun
Publish Year
2020
Experiment Subject
Experiment Type
Cell Experiment
Phenotype Related
Paper Title Cn
Paper Title En
Phagosomal removal of fungal melanin reprograms macrophage metabolism to promote antifungal immunity
Bilingual Status
semi_complete