ReferenceID 2290
Renoprotective Effects of Maslinic Acid on Experimental Renal Fibrosis in Unilateral Ureteral Obstruction Model via Targeting MyD88
Front Pharmacol
Maslinic acid (MA), also named crategolic acid, is a pentacyclic triterpene extracted from fruits and vegetables. Although various beneficial pharmacological effects of MA have been revealed, its effect on renal fibrosis
Relationship Network
Interactive first-hop connections across herbs, ingredients, formulas, targets, diseases, symptoms, syndromes, evidence, and monographs.
Click a node to open it in a new tab
Ingredient: 1Reference: 1Links: 1
Arranging relationship network...
Record Fields
Scalar fields from the final reference record.
- Reference Id
- 2290
- Evidence Id
- 18880
- Core Evidence Id
- 18880
- Source Reference Id
- 4605
- Herb2 Reference Id
- HBREF005402
- Subject Paper Key
- HBIN034527_34588982
- Pubmed Id
- 34588982
- Doi
- 10.3389/fphar.2021.708575
- Paper Title
- Renoprotective Effects of Maslinic Acid on Experimental Renal Fibrosis in Unilateral Ureteral Obstruction Model via Targeting MyD88
- Paper Abstract
- Maslinic acid (MA), also named crategolic acid, is a pentacyclic triterpene extracted from fruits and vegetables. Although various beneficial pharmacological effects of MA have been revealed, its effect on renal fibrosis remains unclear. This study was designed to clarify whether MA could attenuate renal fibrosis and determine the putative underlying molecular mechanisms. We demonstrated that MA-treated mice with unilateral ureteral obstruction (UUO) developed a histological injury of low severity and exhibited downregulated expression of fibrotic markers, including alpha-smooth muscle actin (alpha-SMA), vimentin, and fibronectin by 38, 44 and 40%, and upregulated expression of E-cadherin by 70% as compared with untreated UUO mice. Moreover, MA treatment restored the expression levels of alpha-SMA, connective tissue growth factor, and vimentin to 10, 7.8 and 38% of those induced by transforming growth factor (TGF)-beta in NRK49F cells. MA decreased expression of Smad2/3 phosphorylation and Smad4 in UUO kidneys and TGF-beta treated NRK49F cells (p < 0.05, respectively). Notably, MA specifically interferes with MyD88, an adaptor protein, thereby mitigating Smad4 nuclear expression (p < 0.01 compared to TGF-beta treated group) and ameliorating renal fibrotic changes (p < 0.01 for each fibrotic markers compared to TGF-beta induced cells). In addition, in the UUO model and lipopolysaccharide-induced NRK49F cells, MA treatment decreased the expression of IL-1beta, TGF-alpha and MCP-1, ICAM-1, associated with the suppression of NF-kappaB signaling. These findings suggest that MA is a potential agent that can reduce renal interstitial fibrosis, to some extent, via targeting TGF-beta/Smad and MyD88 signaling.
- Journal
- Front Pharmacol
- Publish Year
- 2021
- Experiment Subject
- mouse; nrk49f cells; tgf-beta induced cells; tgf-beta treated nrk49f cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Renal Fibrosis; Unilateral Ureteral Obstruction; Renal Interstitial Fibrosis
- Paper Title Cn
- Paper Title En
- Renoprotective Effects of Maslinic Acid on Experimental Renal Fibrosis in Unilateral Ureteral Obstruction Model via Targeting MyD88
- Bilingual Status
- semi_complete