ReferenceID 2272
The effects of malvidin on oxidative stress parameters and inflammatory cytokines in LPS-induced human THP-1 cells
J Cell Physiol
Malvidin is an anthocyanin which is involved in inhibiting inflammatory-related mediators in inflammatory diseases; however, its mechanism of action in THP-1 cells is not yet known. THP-1 is a human monocytic cell line t
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- Reference Id
- 2272
- Evidence Id
- 18862
- Core Evidence Id
- 18862
- Source Reference Id
- 4574
- Herb2 Reference Id
- HBREF005371
- Subject Paper Key
- HBIN034359_32914418
- Pubmed Id
- 32914418
- Doi
- 10.1002/jcp.30049
- Paper Title
- The effects of malvidin on oxidative stress parameters and inflammatory cytokines in LPS-induced human THP-1 cells
- Paper Abstract
- Malvidin is an anthocyanin which is involved in inhibiting inflammatory-related mediators in inflammatory diseases; however, its mechanism of action in THP-1 cells is not yet known. THP-1 is a human monocytic cell line that is derived from patients with acute monocytic leukemia. The present study aimed to investigate the effect of malvidin on inflammatory responses and oxidative stress in lipopolysaccharide (LPS)-induced THP-1 cells. THP-1 cells were stimulated with LPS (50 ng/ml) to induce inflammation in the presence or absence of malvidin. The anti/proinflammatory cytokines were evaluated by real-time polymerase chain reaction and enzyme-linked immunosorbent assay. Total protein levels/phosphorylation of c-Jun N-terminal kinase (JNK), P65-NF-kappaB, and IKKalpha/IKKbeta were evaluated by western blot analysis. Malondialdehyde (MDA) and nitric oxide (NO) metabolite levels, ferric reducing antioxidant power (FRAP), total thiol (T-SH) content, and superoxide dismutase (SOD) and glutathione peroxidase (GPx) activity were measured to evaluate the antioxidant activity of malvidin in THP-1 cells. Treatment of LPS-stimulated THP-1 cells with malvidin (100 and 200 muM) led to the significant inhibition of interleukin-6 (IL-6), tumor necrosis factor-alpha, and IL-1beta messenger RNA (mRNA) expression and protein levels as well as a significant increase in the IL-10 mRNA expression and protein secretion. Moreover, 200 muM malvidin treatment reduced the phosphorylation of JNK, IKKalpha/IKKbeta, and P65-NF-kappaB. These findings showed that malvidin not only decreased the MDA and NO metabolite levels but also increased the FRAP and T-SH content as well as SOD and GPx activities. The findings of the present study demonstrated the potential role of malvidin in blocking inflammation and oxidative stress induced by LPS in THP-1 cell line, suggesting that malvidin is likely to be a therapeutic agent for inflammatory diseases.
- Journal
- J Cell Physiol
- Publish Year
- 2021
- Experiment Subject
- human; patient; human monocytic cell line; lipopolysaccharide (lps)-induced thp-1 cells; lps-stimulated thp-1 cells; thp-1 cell line; thp-1 cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Acute Monocytic Leukemia; Inflammatory Diseases
- Paper Title Cn
- Paper Title En
- The effects of malvidin on oxidative stress parameters and inflammatory cytokines in LPS-induced human THP-1 cells
- Bilingual Status
- semi_complete