ReferenceID 2238
Chondroprotective Effect of Cynaroside in IL-1 β-Induced Primary Rat Chondrocytes and Organ Explants via NF- κ B and MAPK Signaling Inhibition
Oxid Med Cell Longev
Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage degradation and inflammation. Interleukin-1beta is the key player in the pathogenesis of OA, which induces the expression of various cataboli
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Record Fields
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- Reference Id
- 2238
- Evidence Id
- 18828
- Core Evidence Id
- 18828
- Source Reference Id
- 4516
- Herb2 Reference Id
- HBREF005313
- Subject Paper Key
- HBIN033853_32047580
- Pubmed Id
- 32047580
- Doi
- 10.1155/2020/9358080
- Paper Title
- Chondroprotective Effect of Cynaroside in IL-1 β-Induced Primary Rat Chondrocytes and Organ Explants via NF- κ B and MAPK Signaling Inhibition
- Paper Abstract
- Osteoarthritis (OA) is a degenerative joint disease characterized by cartilage degradation and inflammation. Interleukin-1beta is the key player in the pathogenesis of OA, which induces the expression of various catabolic factors that contribute to cartilage degradation. Cynaroside (luteolin-7-O-glucoside or luteoloside) is a flavonoid that has various pharmacological properties, such as antitumor, anti-inflammatory, and antioxidant activities. In this study, we investigated the chondroprotective effects of cynaroside on IL-1beta-stimulated chondrocytes and organ explants. The production of nitrite, PGE2, collagen type II, and aggrecan was measured by a Griess reagent and ELISAs, and the production of ROS was measured by H2DCF-DA fluorescence. The protein levels of iNOS, Cox-2, MMP-1, MMP-3, MMP-13, ADAMTS-4, MAPKs, and the NF-kappaB p65 subunit were measured by western blot. Proteoglycan analysis was performed by Alcian Blue staining (in vitro) and Safranin O staining (ex vivo). Cynaroside inhibited IL-1beta-induced expression of catabolic factors (nitrite, iNOS, ROS, PGE2, Cox-2, MMP-1, MMP-3, MMP-13, and ADAMTS-4) and degradation of anabolic factors (collagen type II and aggrecan). Furthermore, cynaroside suppressed IL-1beta-induced phosphorylation of MAPKs and translocation of the NF-kappaB p65 subunit into the nucleus. Collectively, these results suggest that cynaroside may be a potential candidate for the development of new therapeutic drugs for the alleviation of OA progression.
- Journal
- Oxid Med Cell Longev
- Publish Year
- 2020
- Experiment Subject
- Experiment Type
- Animal Experiment
- Phenotype Related
- Inflammation; Degenerative Joint Disease; Osteoarthritis
- Paper Title Cn
- Paper Title En
- Chondroprotective Effect of Cynaroside in IL-1 β-Induced Primary Rat Chondrocytes and Organ Explants via NF- κ B and MAPK Signaling Inhibition
- Bilingual Status
- semi_complete