ReferenceID 2211
The preventive effect of loganin on oxidative stress-induced cellular damage in human keratinocyte HaCaT cells
Biosci Trends
Loganin is a type of iridoid glycosides isolated from Corni fructus and is known to have various pharmacological properties, but studies on its antioxidant activity are still lacking. Therefore, in this study, the preven
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Record Fields
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- Reference Id
- 2211
- Evidence Id
- 18801
- Core Evidence Id
- 18801
- Source Reference Id
- 4461
- Herb2 Reference Id
- HBREF005258
- Subject Paper Key
- HBIN033476_35691912
- Pubmed Id
- 35691912
- Doi
- 10.5582/bst.2022.01116
- Paper Title
- The preventive effect of loganin on oxidative stress-induced cellular damage in human keratinocyte HaCaT cells
- Paper Abstract
- Loganin is a type of iridoid glycosides isolated from Corni fructus and is known to have various pharmacological properties, but studies on its antioxidant activity are still lacking. Therefore, in this study, the preventive effect of loganin on oxidative stress-mediated cellular damage in human keratinocyte HaCaT cells was investigated. Our results show that loganin pretreatment in a non-toxic concentration range significantly improved cell survival in hydrogen peroxide (H 2 O 2 )-treated HaCaT cells, which was associated with inhibition of cell cycle arrest at the G2/M phase and induction of apoptosis. H 2 O 2 -induced DNA damage and reactive oxygen species (ROS) generation were also greatly reduced in the presence of loganin. Moreover, H 2 O 2 treatment enhanced the cytoplasmic release of cytochrome c, upregulation of the Bax/Bcl-2 ratio and degradation of cleavage of poly (ADP-ribose) polymerase, whereas loganin remarkably suppressed these changes. In addition, loganin obviously attenuated H 2 O 2 -induced autophagy while inhibiting the increased accumulation of autophagosome proteins, including as microtubule-associated protein 1 light chain 3-II and Beclin-1, and p62, an autophagy substrate protein, in H 2 O 2 -treated cells. In conclusion, our current results suggests that loganin could protect HaCaT keratinocytes from H 2 O 2 -induced cellular injury by inhibiting mitochondrial dysfunction, autophagy and apoptosis. This finding indicates the applicability of loganin in the prevention and treatment of skin diseases caused by oxidative damage.
- Journal
- Biosci Trends
- Publish Year
- 2022
- Experiment Subject
- human; h 2 o 2 -treated cells; hacat cells; hacat keratinocytes; human keratinocyte hacat cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Skin Diseases; Mitochondrial Dysfunction
- Paper Title Cn
- Paper Title En
- The preventive effect of loganin on oxidative stress-induced cellular damage in human keratinocyte HaCaT cells
- Bilingual Status
- semi_complete