ReferenceID 2203
Lobetyolin induces apoptosis of colon cancer cells by inhibiting glutamine metabolism
J Cell Mol Med
The purpose of the present study was to evaluate the anti-cancer property of Lobetyolin on colorectal cancer and explore its potential mechanism. Lobetyolin was incubated with HCT-116 cells in the absence or presence of
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Record Fields
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- Reference Id
- 2203
- Evidence Id
- 18793
- Core Evidence Id
- 18793
- Source Reference Id
- 4450
- Herb2 Reference Id
- HBREF005247
- Subject Paper Key
- HBIN033455_31990147
- Pubmed Id
- 31990147
- Doi
- 10.1111/jcmm.15009
- Paper Title
- Lobetyolin induces apoptosis of colon cancer cells by inhibiting glutamine metabolism
- Paper Abstract
- The purpose of the present study was to evaluate the anti-cancer property of Lobetyolin on colorectal cancer and explore its potential mechanism. Lobetyolin was incubated with HCT-116 cells in the absence or presence of ASCT2 inhibitor Benser or p53 inhibitor Pifithrin-alpha. The levels of glutamine, glutamic acid, alpha-ketoglutarate, ATP and GSH were determined to measure the glutamine metabolism. Annexin V-FITC/PI staining and TUNEL assay were applied to estimate the apoptotic condition. The levels of ASCT2 were examined by RT-qPCR, Western blot and immunofluorescence staining. The expressions of cleaved-caspase-3, caspase-3, cleaved-caspase-7, caspase-7, cleaved-PARP, PARP, p53, p21, bax and survivin were detected using Western blot analysis. As a result, the treatment with Lobetyolin effectively induced apoptosis and glutamine metabolism in HCT-116 cells through ASCT2 signalling. The inhibition of ASCT2 reduced the glutamine-related biomarkers and augmented the apoptotic process. We further found that the effect of Lobetyolin on HCT-116 was related to the expressions of p21 and bax, and transportation of p53 to nucleus. The inhibition of p53 by Pifithrin-alpha promoted the inhibitory effect of Lobetyolin on ASCT2-mediated apoptosis. Lobetyolin also exerted anti-cancer property in nude mice. In conclusion, the present work suggested that Lobetyolin could induce the apoptosis via the inhibition of ASCT2-mediated glutamine metabolism, which was possibly governed by p53.
- Journal
- J Cell Mol Med
- Publish Year
- 2020
- Experiment Subject
- mouse; hct-116 cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Colorectal Cancer
- Paper Title Cn
- Paper Title En
- Lobetyolin induces apoptosis of colon cancer cells by inhibiting glutamine metabolism
- Bilingual Status
- semi_complete