ReferenceID 2185

Ligustilide promotes apoptosis of cancer-associated fibroblasts via the TLR4 pathways

Food Chem Toxicol

The goal of this research was to study the selective pro-apoptotic effect of ligustilide on prostate-cancer-associated fibroblast in the tumor microenvironment and the related molecular mechanisms. The effects of ligusti

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Reference Id
2185
Evidence Id
18775
Core Evidence Id
18775
Source Reference Id
4417
Herb2 Reference Id
HBREF005214
Subject Paper Key
HBIN033198_31765699
Pubmed Id
31765699
Doi
10.1016/j.fct.2019.110991
Paper Title
Ligustilide promotes apoptosis of cancer-associated fibroblasts via the TLR4 pathways
Paper Abstract
The goal of this research was to study the selective pro-apoptotic effect of ligustilide on prostate-cancer-associated fibroblast in the tumor microenvironment and the related molecular mechanisms. The effects of ligustilide on cancer-associated fibroblasts (CAFs) and normal fibroblasts (NFs) isolated from the prostate were determined by MTT assay. Flow cytometry and cellular immunofluorescence were used to detect the effects of ligustilide on the cell cycle and apoptosis. Western blotting was used to detect the expression of apoptosis-related proteins after the action of ligustilide on CAFs. In the investigation, ligustilide had a selective pro-apoptotic effect on prostate-CAFs. After ligustilide treatment, the proportion of CAFs in the G2-M phase of the cell cycle increased, and the expression of apoptosis-related proteins (p-P53, Bcl-2, Caspase9 and Cytochrome C) changed. Ligustilide blocks the CAF cell cycle and induces the apoptosis of CAFs.
Journal
Food Chem Toxicol
Publish Year
2020
Experiment Subject
Experiment Type
Cell Experiment
Phenotype Related
Tumor
Paper Title Cn
Paper Title En
Ligustilide promotes apoptosis of cancer-associated fibroblasts via the TLR4 pathways
Bilingual Status
semi_complete