ReferenceID 2174
Homocysteine induces oxidative stress and ferroptosis of nucleus pulposus via enhancing methylation of GPX4
Free Radic Biol Med
Homocysteine (Hcy) is an amino acid involved in gene methylation. Plasma concentration of Hcy is elevated in the pathological condition hyperhomocysteinemia (HHcy), which increases the risk of disorders of the vascular,
Relationship Network
Interactive first-hop connections across herbs, ingredients, formulas, targets, diseases, symptoms, syndromes, evidence, and monographs.
Click a node to open it in a new tab
Ingredient: 1Reference: 1Links: 1
Arranging relationship network...
Record Fields
Scalar fields from the final reference record.
- Reference Id
- 2174
- Evidence Id
- 18764
- Core Evidence Id
- 18764
- Source Reference Id
- 4401
- Herb2 Reference Id
- HBREF005198
- Subject Paper Key
- HBIN033053_32896601
- Pubmed Id
- 32896601
- Doi
- 10.1016/j.freeradbiomed.2020.08.029
- Paper Title
- Homocysteine induces oxidative stress and ferroptosis of nucleus pulposus via enhancing methylation of GPX4
- Paper Abstract
- Homocysteine (Hcy) is an amino acid involved in gene methylation. Plasma concentration of Hcy is elevated in the pathological condition hyperhomocysteinemia (HHcy), which increases the risk of disorders of the vascular, nervous and musculoskeletal systems, including chondrocyte dysfunction. The present study aimed to explore the role of Hcy in intervertebral disc degeneration (IVDD), using a range of techniques. A clinical epidemiological study showed that HHcy is an independent risk factor for human IVDD. Cell culture using rat nucleus pulposus cells showed that Hcy promotes a degenerative cell phenotype (involving increased oxidative stress and cell death by ferroptosis) which is mediated by upregulated methylation of GPX4. An in-vivo mouse 'puncture' model of IVDD showed that folic acid (which is used to treat HHcy in humans) reduced the ability of diet-induced HHcy to promote IVDD. We conclude that Hcy upregulates oxidative stress and ferroptosis in the nucleus pulposus via enhancing GPX4 methylation, and is a new contributing factor in IVDD.
- Journal
- Free Radic Biol Med
- Publish Year
- 2020
- Experiment Subject
- mouse; rat; human
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Chondrocyte Dysfunction; Hhcy; Hyperhomocysteinemia; Disorders Of The Vascular, Nervous And Musculoskeletal Systems; Intervertebral Disc Degeneration
- Paper Title Cn
- Paper Title En
- Homocysteine induces oxidative stress and ferroptosis of nucleus pulposus via enhancing methylation of GPX4
- Bilingual Status
- semi_complete