ReferenceID 2128
Isorhynchophylline ameliorates the progression of osteoarthritis by inhibiting the NF-κB pathway
Eur J Pharmacol
Osteoarthritis (OA), a progressive and degenerative joint disease, is characterized by cartilage degradation, synovitis, subchondral bone remodeling and osteophyte formation. Isorhynchophylline (IRN) is an oxindole alkal
Relationship Network
Interactive first-hop connections across herbs, ingredients, formulas, targets, diseases, symptoms, syndromes, evidence, and monographs.
Click a node to open it in a new tab
Ingredient: 1Reference: 1Links: 1
Arranging relationship network...
Record Fields
Scalar fields from the final reference record.
- Reference Id
- 2128
- Evidence Id
- 18718
- Core Evidence Id
- 18718
- Source Reference Id
- 4290
- Herb2 Reference Id
- HBREF005087
- Subject Paper Key
- HBIN031193_35469836
- Pubmed Id
- 35469836
- Doi
- 10.1016/j.ejphar.2022.174971
- Paper Title
- Isorhynchophylline ameliorates the progression of osteoarthritis by inhibiting the NF-κB pathway
- Paper Abstract
- Osteoarthritis (OA), a progressive and degenerative joint disease, is characterized by cartilage degradation, synovitis, subchondral bone remodeling and osteophyte formation. Isorhynchophylline (IRN) is an oxindole alkaloid isolated from the traditional Chinese herb Uncaria rhynchophylla. In this study, we evaluated the protective effects of IRN on human OA chondrocytes. IRN treatment dose-dependently decreased the interleukin-1β (IL-1β)-induced expressions of nitric oxide (NO; p < 0.001), prostaglandin E2 (PGE2; p < 0.001), tumor necrosis factor alpha (TNF-α; p < 0.001), interleukin-6 (IL-6; p < 0.001), cyclooxygenase-2 (COX-2; p < 0.001) and inducible nitric oxide synthase (iNOS; p < 0.001) in chondrocytes. Meanwhile, the production of metalloproteinase 13 (MMP13; p < 0.001) and a disintegrin and metalloproteinase with thrombospondin motifs 5 (ADAMTS5; p < 0.001) was inhibited by IRN treatment. Molecular docking studies revealed that IRN directly interacted with the nuclear factor kappa B (NF-κB) complex, which was associated with a reduced level of NF-κB nuclear translocation and the inhibition of NF-κB signaling activity. Furthermore, administration of IRN generated marked in vivo protective effects during OA development. Collectively, our results demonstrate that IRN may exhibit therapeutic benefits against OA, potentially by ameliorating the inflammative and degenerative progression of OA via inhibiting the NF-κB pathway.
- Journal
- Eur J Pharmacol
- Publish Year
- 2022
- Experiment Subject
- human
- Experiment Type
- Cell Experiment
- Phenotype Related
- Degenerative Joint Disease; Subchondral Bone Remodeling; Tumor; Osteoarthritis; Osteophyte; Synovitis
- Paper Title Cn
- Paper Title En
- Isorhynchophylline ameliorates the progression of osteoarthritis by inhibiting the NF-κB pathway
- Bilingual Status
- semi_complete