ReferenceID 2127

Isorhynchophylline Ameliorates Cerebral Ischemia/Reperfusion Injury by Inhibiting CX3CR1-Mediated Microglial Activation and Neuroinflammation

Front Pharmacol

Reperfusion therapy is an effective way to rescue cerebral ischemic injury, but this therapy also shows the detrimental risk of devastating disorders and death due to the possible inflammatory responses involved in the p

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Reference Id
2127
Evidence Id
18717
Core Evidence Id
18717
Source Reference Id
4289
Herb2 Reference Id
HBREF005086
Subject Paper Key
HBIN031193_33643044
Pubmed Id
33643044
Doi
10.3389/fphar.2021.574793
Paper Title
Isorhynchophylline Ameliorates Cerebral Ischemia/Reperfusion Injury by Inhibiting CX3CR1-Mediated Microglial Activation and Neuroinflammation
Paper Abstract
Reperfusion therapy is an effective way to rescue cerebral ischemic injury, but this therapy also shows the detrimental risk of devastating disorders and death due to the possible inflammatory responses involved in the pathologies. Hence, the therapy of ischemia/reperfusion (I/R) injury is a great challenge currently. Isorhynchophylline (IRN), a tetracyclic oxindole alkaloid extracted from Uncaria rhynchophylla, has previously shown neuroprotective and anti-inflammatory effects in microglial cells. This study systematically investigates the effect of IRN on I/R injury and its underlying mechanism. The effects of IRN on neuronal injury and microglia-mediated inflammatory response were assessed on a rat model with middle cerebral artery occlusion (MCAO) and reperfusion-induced injury. We found that IRN treatment attenuated the infarct volume and improved the neurological function in I/R injury rats. IRN treatment also reduced the neuronal death rate, brain water content, and aquaporin-4 expression in the ischemic penumbra of I/R injury rats' brains. Besides, IRN treatment could inhibit the following process, including IkappaB-alpha degradation, NF-kappaB p65 activation, and CX3CR1 expression, as well as the microglial activation and inflammatory response. These findings suggest that IRN is a promising candidate to treat the cerebral I/R injury via inhibiting microglia activation and neuroinflammation.
Journal
Front Pharmacol
Publish Year
2021
Experiment Subject
rat
Experiment Type
Animal Experiment
Phenotype Related
Cerebral Ischemic Injury; Cerebral I/r Injury; I/r Injury; Middle Cerebral Artery Occlusion; Ischemia/reperfusion (i/r) Injury
Paper Title Cn
Paper Title En
Isorhynchophylline Ameliorates Cerebral Ischemia/Reperfusion Injury by Inhibiting CX3CR1-Mediated Microglial Activation and Neuroinflammation
Bilingual Status
semi_complete