ReferenceID 2039

Hesperetin Inhibits Expression of Virulence Factors and Growth of Helicobacter pylori

Int J Mol Sci

Helicobacter pylori (H. pylori) is a bacterium known to infect the human stomach. It can cause various gastrointestinal diseases including gastritis and gastric cancer. Hesperetin is a major flavanone component contained

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Reference Id
2039
Evidence Id
18629
Core Evidence Id
18629
Source Reference Id
4098
Herb2 Reference Id
HBREF004895
Subject Paper Key
HBIN029190_34576198
Pubmed Id
34576198
Doi
10.3390/ijms221810035
Paper Title
Hesperetin Inhibits Expression of Virulence Factors and Growth of Helicobacter pylori
Paper Abstract
Helicobacter pylori (H. pylori) is a bacterium known to infect the human stomach. It can cause various gastrointestinal diseases including gastritis and gastric cancer. Hesperetin is a major flavanone component contained in citrus fruits. It has been reported to possess antibacterial, antioxidant, and anticancer effects. However, the antibacterial mechanism of hesperetin against H. pylori has not been reported yet. Therefore, the objective of this study was to determine the inhibitory effects of hesperetin on H. pylori growth and its inhibitory mechanisms. The results of this study showed that hesperetin inhibits the growth of H. pylori reference strains and clinical isolates. Hesperetin inhibits the expression of genes in replication (dnaE, dnaN, dnaQ, and holB) and transcription (rpoA, rpoB, rpoD, and rpoN) machineries of H. pylori. Hesperetin also inhibits the expression of genes related to H. pylori motility (flhA, flaA, and flgE) and adhesion (sabA, alpA, alpB, hpaA, and hopZ). It also inhibits the expression of urease. Hespereti n downregulates major virulence factors such as cytotoxin-associated antigen A (CagA) and vacuolating cytotoxin A (VacA) and decreases the translocation of CagA and VacA proteins into gastric adenocarcinoma (AGS) cells. These results might be due to decreased expression of the type IV secretion system (T4SS) and type V secretion system (T5SS) involved in translocation of CagA and VacA, respectively. The results of this study indicate that hesperetin has antibacterial effects against H. pylori. Thus, hesperetin might be an effective natural product for the eradication of H. pylori.
Journal
Int J Mol Sci
Publish Year
2021
Experiment Subject
human
Experiment Type
Cell Experiment
Phenotype Related
Helicobacter Pylori; Gastrointestinal Diseases; Gastric Cancer; Gastritis; Gastric Adenocarcinoma
Paper Title Cn
Paper Title En
Hesperetin Inhibits Expression of Virulence Factors and Growth of Helicobacter pylori
Bilingual Status
semi_complete