ReferenceID 2009
Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte
Antioxidants (Basel)
This study aimed to investigate the anti-inflammatory, antioxidant, and anti-atopic dermatitis (AD) effects of haplopine, which is one of the active components in D. dasycarpus. Haplopine (12.5 and 25 muM) inhibited the
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Record Fields
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- Reference Id
- 2009
- Evidence Id
- 18599
- Core Evidence Id
- 18599
- Source Reference Id
- 4029
- Herb2 Reference Id
- HBREF004826
- Subject Paper Key
- HBIN028793_34069696
- Pubmed Id
- 34069696
- Doi
- 10.3390/antiox10050806
- Paper Title
- Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte
- Paper Abstract
- This study aimed to investigate the anti-inflammatory, antioxidant, and anti-atopic dermatitis (AD) effects of haplopine, which is one of the active components in D. dasycarpus. Haplopine (12.5 and 25 muM) inhibited the mRNA expressions of inflammatory cytokines IL-6, TSLP, GM-CSF, and G-CSF and the protein expressions of IL-6 and GM-CSF in TNF-alpha/INF-gamma-stimulated HaCaT cells. In H2O2-induced Jukat T cells, haplopine (25 and 50 muM) suppressed the productions of proinflammatory cytokines (IL-4, IL-13, and COX-2) and increased the mRNA and protein expressions of oxidative stress defense enzymes (SOD, CAT, and HO-1) in a concentration-dependent manner. In vivo, haplopine significantly attenuated the development of AD symptoms in 2,4-dinitrochlorobenzene (DNCB)-stimulated Balb/c mice, as evidenced by reduced clinical dermatitis scores, skin thickness measurements, mast cell infiltration, and serum IgE concentrations. These findings demonstrate that haplopine should be considered a novel anti-atopic agent with the potential to treat AD.
- Journal
- Antioxidants (Basel)
- Publish Year
- 2021
- Experiment Subject
- mouse; h2o2-induced jukat t cells; tnf-alpha/inf-gamma-stimulated hacat cells
- Experiment Type
- Animal & Cell Experiment
- Phenotype Related
- Anti-atopic Dermatitis; Dermatitis
- Paper Title Cn
- Paper Title En
- Haplopine Ameliorates 2,4-Dinitrochlorobenzene-Induced Atopic Dermatitis-Like Skin Lesions in Mice and TNF-α/IFN-γ-Induced Inflammation in Human Keratinocyte
- Bilingual Status
- semi_complete