ReferenceID 1997
Effect of gomisin A on osteoblast differentiation in high glucose-mediated oxidative stress
Phytomedicine
BACKGROUND: Gomisin A is a lignan isolated from the hexane of Schisandra chinensis fruit extract with antioxidant properties. Oxidative stress mediated by high glucose is one of the major complications of diabetes mellit
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 1997
- Evidence Id
- 18587
- Core Evidence Id
- 18587
- Source Reference Id
- 4013
- Herb2 Reference Id
- HBREF004810
- Subject Paper Key
- HBIN028273_31790903
- Pubmed Id
- 31790903
- Doi
- 10.1016/j.phymed.2019.153107
- Paper Title
- Effect of gomisin A on osteoblast differentiation in high glucose-mediated oxidative stress
- Paper Abstract
- BACKGROUND: Gomisin A is a lignan isolated from the hexane of Schisandra chinensis fruit extract with antioxidant properties. Oxidative stress mediated by high glucose is one of the major complications of diabetes mellitus. PURPOSE: This study investigates the role of gomisin A in osteoblast differentiation under high glucose-induced oxidative stress in MC3T3 E1 cells and determines its relationship with heme oxygenase-1 (HO-1) and mitochondrial biogenesis. METHODS: MC3T3 E1 cells were treated by gomisin A following induced by high glucose levels and glucose oxidase to investigate the inhibitory effect of gomisin A against high glucose oxidative stress. Western blot analysis, alizarin red staining, alkaline phosphatase (ALP) activity, analysis of reactive oxygen species (ROS) and confocal microscopy were used to determine mitochondrial biogenesis, oxidative stress, osteoblast differentiation and mineralization. To analyze the role of HO-1, the MC3T3 E1 cells were treated with the HO-1 inhibitor zinc protoporphyrin IX (ZnPP). RESULTS: Gomisin A enhanced the expression of HO-1, increased mitochondrial biogenesis factors (peroxisome proliferator-activated receptor gamma coactivator 1-alpha, nuclear respiratory factor-1, and mitochondrial transcription factor A), antioxidant enzymes (copper-zinc superoxide dismutases and manganese superoxide dismutase), osteoblast differentiation molecules (bone morphogenic protein-2/7, osteoprotegerin and Runt-related transcription factor-2) and mineralization by upregulation of ALP and alizarin red staining, which were decreased by ZnPP and high glucose oxidative stress. Similarly, gomisin A inhibited ROS which was increased by ZnPP and the high glucose-mediated oxidative stress. CONCLUSIONS: The findings demonstrated the antioxidative effects of gomisin A, and its role in mitochondrial biogenesis and osteoblast differentiation. It potentially regulated osteoblast differentiation under high glucose-induced oxidative stress via upregulation of HO-1 and maintenance of mitochondrial homeostasis. Thus, gomisin A may represent a potential therapeutic agent for prevention of bone fragility fractures and implant failure triggered by diabetes.
- Journal
- Phytomedicine
- Publish Year
- 2020
- Experiment Subject
- mc3t3 e1 cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Diabetes; Diabetes Mellitus; Bone Fragility Fractures; Implant Failure
- Paper Title Cn
- Paper Title En
- Effect of gomisin A on osteoblast differentiation in high glucose-mediated oxidative stress
- Bilingual Status
- semi_complete