ReferenceID 1983
SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells
Nature
Glutathione (GSH) is a small-molecule thiol that is abundant in all eukaryotes and has key roles in oxidative metabolism1. Mitochondria, as the major site of oxidative reactions, must maintain sufficient levels of GSH to
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Record Fields
Scalar fields from the final reference record.
- Reference Id
- 1983
- Evidence Id
- 18573
- Core Evidence Id
- 18573
- Source Reference Id
- 3988
- Herb2 Reference Id
- HBREF004785
- Subject Paper Key
- HBIN028061_34707288
- Pubmed Id
- 34707288
- Doi
- 10.1038/s41586-021-04025-w
- Paper Title
- SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells
- Paper Abstract
- Glutathione (GSH) is a small-molecule thiol that is abundant in all eukaryotes and has key roles in oxidative metabolism1. Mitochondria, as the major site of oxidative reactions, must maintain sufficient levels of GSH to perform protective and biosynthetic functions2. GSH is synthesized exclusively in the cytosol, yet the molecular machinery involved in mitochondrial GSH import remains unknown. Here, using organellar proteomics and metabolomics approaches, we identify SLC25A39, a mitochondrial membrane carrier of unknown function, as a regulator of GSH transport into mitochondria. Loss of SLC25A39 reduces mitochondrial GSH import and abundance without affecting cellular GSH levels. Cells lacking both SLC25A39 and its paralogue SLC25A40 exhibit defects in the activity and stability of proteins containing iron-sulfur clusters. We find that mitochondrial GSH import is necessary for cell proliferation in vitro and red blood cell development in mice. Heterologous expression of an engineered bifunctional bacterial GSH biosynthetic enzyme (GshF) in mitochondria enables mitochondrial GSH production and ameliorates the metabolic and proliferative defects caused by its depletion. Finally, GSH availability negatively regulates SLC25A39 protein abundance, coupling redox homeostasis to mitochondrial GSH import in mammalian cells. Our work identifies SLC25A39 as an essential and regulated component of the mitochondrial GSH-import machinery.
- Journal
- Nature
- Publish Year
- 2021
- Experiment Subject
- mouse
- Experiment Type
- Cell Experiment
- Phenotype Related
- Paper Title Cn
- Paper Title En
- SLC25A39 is necessary for mitochondrial glutathione import in mammalian cells
- Bilingual Status
- semi_complete