ReferenceID 1796

Echinacoside Protects Dopaminergic Neurons Through Regulating IL-6/JAK2/STAT3 Pathway in Parkinson's Disease Model

Front Pharmacol

Echinacoside (ECH), the major active constituent of Cistanche deserticola , was found to exert neuroprotection through neurotrophic and anti-inflammatory functions in Parkinson's disease (PD) models. However, a clear int

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Reference Id
1796
Evidence Id
18386
Core Evidence Id
18386
Source Reference Id
3580
Herb2 Reference Id
HBREF004377
Subject Paper Key
HBIN024794_35281889
Pubmed Id
35281889
Doi
10.3389/fphar.2022.848813
Paper Title
Echinacoside Protects Dopaminergic Neurons Through Regulating IL-6/JAK2/STAT3 Pathway in Parkinson's Disease Model
Paper Abstract
Echinacoside (ECH), the major active constituent of Cistanche deserticola , was found to exert neuroprotection through neurotrophic and anti-inflammatory functions in Parkinson's disease (PD) models. However, a clear intermediate molecule or pathway that unifies these two effects has to be found. In this study, our results demonstrate that ECH can protect DA neurons in PD mice with Western blot and immunohistochemistry staining. The quantitative real-time polymerase chain reaction was adapted to confirm its anti-inflammatory function with decreased cytokines (interleukin- (IL-) 6, IL-1β, and TNF-α) in PD mice and LPS-induced BV2 cells. Further studies found that ECH inhibited the IL-6/JAK2/STAT3 pathway and decreased phosphorylation of STAT3 on tyr705 by Western blot. It can also increase p-STAT3 (ser727) and brain-derived neurotrophic factor (BDNF) expression in PD mice and LPS-induced BV2 cells. This study revealed that ECH exerts neurotrophic and anti-inflammatory effects by regulating the IL-6/JAK2/STAT3 pathway and the phosphorylation of STAT3, promoting the mutually beneficial influence of the two effects to maximize its neuroprotective function.
Journal
Front Pharmacol
Publish Year
2022
Experiment Subject
mouse; bv2 cells
Experiment Type
Animal & Cell Experiment
Phenotype Related
Parkinson's Disease
Paper Title Cn
Paper Title En
Echinacoside Protects Dopaminergic Neurons Through Regulating IL-6/JAK2/STAT3 Pathway in Parkinson's Disease Model
Bilingual Status
semi_complete