ReferenceID 1763
Diallyl disulfide suppresses the lipopolysaccharide-driven inflammatory response of macrophages by activating the Nrf2 pathway
Food Chem Toxicol
Lipopolysaccharide (LPS)-driven activation of Kupffer cells plays critical roles in the development of alcoholic liver disease (ALD). Accumulating evidence has revealed that nuclear factor erythroid 2-related factor 2 (N
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Record Fields
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- Reference Id
- 1763
- Evidence Id
- 18353
- Core Evidence Id
- 18353
- Source Reference Id
- 3503
- Herb2 Reference Id
- HBREF004300
- Subject Paper Key
- HBIN023556_34896185
- Pubmed Id
- 34896185
- Doi
- 10.1016/j.fct.2021.112760
- Paper Title
- Diallyl disulfide suppresses the lipopolysaccharide-driven inflammatory response of macrophages by activating the Nrf2 pathway
- Paper Abstract
- Lipopolysaccharide (LPS)-driven activation of Kupffer cells plays critical roles in the development of alcoholic liver disease (ALD). Accumulating evidence has revealed that nuclear factor erythroid 2-related factor 2 (Nrf2) can modulate the polarization of macrophages. The current study aimed to investigate the roles of diallyl disulfide (DADS) in LPS-driven inflammation in vitro and in vivo. We found that DADS significantly increased the nuclear translocation of Nrf2 and the transcription of Nrf2 targets, including HO1, NQO1, and gamma-GCSc, and suppressed degradation of Nrf2 protein. Besides, DADS significantly inhibited LPS-induced activation of NF-kappaB and MAPK, secretion of NO and TNF-alpha, and production of reactive oxygen species (ROS) in LPS-exposed RAW264.7 cells. In vivo study demonstrated that DADS significantly ameliorated liver damage in mice challenged with LPS, as shown by the inhibition of increases in serum aminotransferase activities, neutrophil infiltration, and NF-kappaB and NLRP3 inflammasome activation. Finally, knockout of Nrf2 abrogated the suppression of DADS on macrophage polarization and on liver injury induced by LPS. These findings reveal that DADS suppresses LPS-driven inflammatory response in the liver by activating Nrf2, which suggests that the protective effects of DADS against ALD may be attributed to the modulation of Kupffer cell polarization in the liver.
- Journal
- Food Chem Toxicol
- Publish Year
- 2022
- Experiment Subject
- mouse; raw264.7 cells
- Experiment Type
- Cell Experiment
- Phenotype Related
- Alcoholic Liver Disease
- Paper Title Cn
- Paper Title En
- Diallyl disulfide suppresses the lipopolysaccharide-driven inflammatory response of macrophages by activating the Nrf2 pathway
- Bilingual Status
- semi_complete